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1、Source:http://www.science-truth.com植物学通报2005,22(6):703~707ChineseBulletinofBotany实验简报沙棘果皮多糖清除氧自由基的活性研究①②张海容(山西省忻州师范学院生化分析技术研究所忻州034000)摘要沙棘(Hippophaerhamnosides)果皮经80℃恒温水浴提取,乙醇沉淀得粗多糖。Sevag法去蛋白,2+经50%和70%乙醇分级,得3种级分沙棘多糖H1、H2和H3;以Fenton反应,即H2O2/Fe/水杨酸为.OH产-.生和检测体系;以邻苯
2、三酚/EDTA/Tris-HCl为O2产生体系,对沙棘多糖H1、H2和H3进行抗氧自由基活-.性研究。结果表明,沙棘多糖对.OH和O2有较显著的清除能力。不同级分多糖H1、H2和H3浓度达200mg.mL-1时,对.OH的清除率分别为44.9%、49.0%和26.4%,抗O-.活性分别为36.9%,15.4%和23.1%。多2糖质量浓度增大时,两种自由基清除率增加,且呈量效关系。关键词沙棘,多糖,超氧阴离子,羟自由基,自由基清除率StudyoftheScavengingRadicalActivitiesofPolysacch
3、arideofHippophaerhamnosidesFruitPeel②ZHANGHai-Rong(InstituteofBiochemicalAnalysis,XinzhouTeachersUniversity,Xinzhou034000)AbstractTostudythescaventingradicalactivitiesofpolysaccharideofHippophaerhamnodidesfruitpeel,crudepolysaccharidewasisolatedfromthefruitpeelofH.
4、rhamnosideswithhotwaterat80℃,thenprecipitatedbyethanol,andtheproteinintheextractswasremovedbytheSevagmethod.ThefractionsofH1、H2andH3ofH.rhamnosidespolysaccharidewereobtainedbyadding50%and70%ethanol,respectively.TheFentonreactionwasusedtoproduceanddetecthydroxylradi
5、cals(.OH),andpyrogallol/EDTA/Tris-HClwasusedtoproduceanddetectsuper--.oxideanion(O2).H.rhamnosidespolysaccharideshadastrongscavengingcapacityforboth.OHandO-.oxygenradicals.WhenthefractionalconcentrationsofH、HandHwereat2002123-1mg.mL,theirscavengingratestohydroxylra
6、dicalswere44.9%,49.0%,and26.4%,respectively,andtosuperoxideanion36.9%,15.4%,and23.1%,respectively.Thescavengingratestooxygenradicalsincreasedwithincreasingthepolysaccharideconcentration;thereappearedapropor-tionalrelationbetweeneffectandquantity.KeywordsHippophaerh
7、amnosides,Polysaccharide,Superoxideanionfreeradical,Hydroxylradicals,Freeradicalscavengingrate①山西省归国留学基金(2004-67)资助。②通讯作者。Authorforcorrespondence.E-mail:biochem@xztc.edu.cn收稿日期:2005-01-18接受日期:2005-04-30责任编辑:于昕www.science-truth.comSource:http://www.science-truth.com
8、70422(6)-.氧自由基主要指超氧阴离子(O2)、羟自1.1材料与仪器1由基(.OH)、单线态氧(O2)和过氧化氢(H2O2)沙棘果皮由山西岢岚县酸溜溜厂提供(10一类化学性质活泼、半衰期极短、能夺取其月下旬)。他敏感分子电子的氧化剂。氧自由基过多的DKZ电热恒温振荡水槽,上海一恒实验
