s100a4基因在脑胶质瘤中的表达与意义

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1、硕士学位论文中文摘要重要的依据，有望成为脑胶质瘤新的分子生物标记和基因靶向治疗的靶点。关键词：脑胶质瘤，S100A4，免疫组化，RT-PCR‘篱：。一：’_-燮堡笙兰墨兰塑矍ABSTRACTObjective：ByanalyingtheexpressionofS100A4inmRNAandproteinofdifferentpathologicalgradeofglioma，soastoprovidetheoreticalbasisfortheexplorationofthemechanisminwhich

2、waycouldinducedevelopinggliomaMethod：Inourexperiment，wecollected59casesoffreshhumangliomaspecimens，12gasesofnormalbraintissuespecimensand5casesofbenignintracranialfromNeurosurgeryinXiangyaHospitsl，CentralSouthUniversityin2010-2011Thetissuesamplesfromglioma

3、parlentsunderwentinitialsurgeryandconfirmedbypathology，thesepatientshadnotreceivedchemotherapybeforesurgeryandhadcompleterelatedclinicaldata：thegliomatissue49cases；normalbraintissue12cases：benignintracranialtussue5forexampleRT-PCRandImmunohistochemicalmeth

4、od(SP)wereusedtodetectmRNAandtheproteinoftheexpressionofSl00A4，theapplicationoftheImageJandM／ASmedicalimageanalysissystemsoftwaretoanalysisSPSSl70sothvareforstatisticalanalysisResults：RT．PCRanalysisshowedthattheexpressionofSl00A4mRNAinthenormalbraintissue，

5、intracranialbenigntumor，andthehumallbrain硕士学位论文英文摘要gliomaWHOI，WHOII，WHOIll，WHOIVare0019±0007，00297±0008，01333±0012，01847100133，0486±0011，05083±0017．Theexpressionof9100A4intheintracranialbenigntumorgroupandnormalgrouphadnodifference．(p>O05)Buttheexpressiono

6、fS100A4mRNAinthegroupofnormalhumanbraintogliomagradeI／1I，Ill／1VwasgraduallyincreasingfP<0．05)．WiththeincreasingofgliomasWHOlevel．S100A4expressiongraduallystrengthenedImmunohisto—chemicalanalysisshowedthatS100A4wasexpressingmainlyinthecytoplasmTheexpression

7、rateofS100A4inthenormaIbraintissue，intracranialbenigntumor，andthehumanbraingliomaWHOi，WHOII，WHOIII．WHOIVis83％。20O％，333％，412％，864％．909％．TheexpressionofSl00A4intheintracranialbenigntumorgroupandnormalgrouphavenodifferenceButtheexpressionofS100A4inthegroupofn

8、ormalhumanbraintOgliomagradeI／II，IlI／1Vwasgraduallyincreasing(p<005)WiththeincreasingofgliomasWHOlevel．S100A4expressionwasgraduallystrengtheningConelusion：1．TheexpressionofS100A4issignificantlyupregulatedinpr