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1、第三军医大学硕士学位论文numberoftargetednanobubblesadheredtoLNCaPcellsandC4-2cells.Incontrast,nosignificantadhesionoftargetednanobubblewasobservedaroundMKN45cells.NoadhesionofblanknanobubblewasobservedaroundLNCaPcells,C4-2cellsorMKN45cells.PreparationofPSMA-targetednano
2、bubblewithelectrostaticadsorption:theaverage8particlesizewas(702.96±66.65)nm,concentrationwasabout(3.46±0.3)x10/ml,thepH4.6solutionwasmostefficient,andprostatecancercellsLNCaPadhesionratewas(77.2±3.19)%,andC4-2celladhesionratewas(61±4.47)%;whilepreparationof
3、PSMA-targetednanobubblewithavidinbiotincomplextechnology：theaverageparticlesize8was(609.90±37.40)nm,concentrationwasabout(4.52±0.19)x10/ml,andprostatecancercellsLNCaPadhesionratewas(96.6±1.14)%,andC4-2celladhesionratewas(94±1.58)%.Theparticlesize,concentrati
4、onandcelladhesionrateofthetwokindsoftargetedmicrobubblewerestatisticallysignificant.3.Threekindsoftransplantedtumorsinnudemiceweresuccessfullyestablished.Therewasnosignificantdifferencebetweenthetargetedandblanknanobubblesinthearrivaltimeorthetimetopeak(P>0.
5、05)inthreetransplantedprostatetumors,butthepeakintensityandthedurationofcontrastenhancementweresignificantlydifferentinbothtransplantedprostatetumors(P＜0.05),notinthetransplantedMKN45tumors(P>0.05).Amongthethreetypesoftransplantedtumorswithtargetednanobubble
6、s,thepeakintensityinprostatetumors(LNCaPandC4-2)wassignificantlyhigherthanthatingastrictumors(MKN45)(P＜0.05).Conclusion:1.CellimmunofluorescenceindicatesthatLNCaPcellsandC4-2cellshaveahighPSMAexpression,whileMKN45cellshavenoPSMAexpression.2.Theelectrostatica
7、bsorptionmethodandavidinbiotincomplextechnologycouldbebothusedtopreparetargetednanobubbles.TheycouldbindspecificallywithprostatecancercellsLNCaP,C4-2,andnotargetedbindingwithMKN45cells.PSMA-targetednanobubblespreparedbyavidinbiotincomplextechnologyarebettert
8、hanthosepreparedbyelectrostaticabsorptionmethod.Theformerhasthesmallerparticlesize,thehigherconcentration,thegreaterstability,andthebettertargetingcapabilityofprostatecancercells.3.ThePSMA-targe
