klotho蛋白、caspase12蛋白在糖尿病肾病中的表达与意义

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1、万方数据英文摘要Klothoproteinisprimarilyexpressedinrenalproximaltubules．Byimmunohistochemistrystaining，thepositivereactionofKlothowasidentifiedincytoplasm，characterizedbybrown—yellowishgranulesincytoplasm．Therewaspositivebasalexpressionintherenalproximaltubularepithelialcellsinthecontrolgro

2、up．TheexpressionofKlothoinDNgroupweresignificantlylowerthanthatofcontrolgroup(P<0．05)．Caspase一12proteinisprimarilyexpressedinrenalglomeruliandtubules．Byimmunohistochemistry,thepositivereactionofcaspase-12proteinwasidentifiedincellnucleusand／orcytoplasm，representedbybrown—yellowishgr

3、anulesinthecytoplasmand／ornuclear．Therewaslightpositivebasalexpressioninthecontrolgroup．IntheDNgroup，thenumbersofpositive--expressedcellsandtheexpressionintensityofCaspase--12inrenaltissueweresignificantlyhigherthanthatofcontrolgroup衅0．01，氏O．05)．Accordingtothecorrelationanalysis，the

4、reisanegativecorrelationbetweentheexpressionofKlothoandcaspase-12(F一0．643，P<0．05)inratswithDN．4TheapoptosisoftubularepithelialcellsbyTUNELstainingByTUNELstaining，theapoptoticcellswereidentifiedbybrown-yellowishgranulesincellnucleus．Theapoptoticcellswerecountedtocalculatetheapoptotic

5、index．TheapoptoticindexoftubularepithelialcellsinDNgroup(34．64-5．2)wassignificantlyhigherthanthatofcontrolgroup(1．6土1．2)仔k0．01)．Conclusion：IthasshownthattheexpressionofKlothoproteinWasinhibitedandtheexpressionofCaspase一12proteinwasincreasedinrenaltubulesofratswithtype2diabetes．There

6、isanegativecorrelationbetweentheexpressionofKlothoandcaspase-12inratswithDN．Meanwhile，theapoptosisinrenaltubulescellsincreased．ItisproposedthatapoptosismediatedbyERstressmightbeinvolvedinthepathogenesisanddevelopmentofDN．Keywords：Type2diabetesmellitus，diabeticnephropathy,Klotho，Casp

7、ase一12，renaltubularepithelialcell，apoptosis7万方数据研究论文Klotho蛋白、Caspasel2蛋白在糖尿病肾病中的表达及意义前言糖尿病已成为全球性疾病，在世界人口死亡原因中位居第五位。我国的糖尿病患病率在10年期间增长了4倍以上，已经攀升到了将近10％，成为世界上发病最多的国家。2009年国际糖尿病联盟公布数据显示全世界已有2．85亿糖尿病患者，预计2030年达到4．35亿。糖尿病肾病是糖尿病最常见的并发症之一，愈来愈威胁着糖尿病患者的生存，成为终末期肾功能衰竭的主要原因之一。糖尿病肾病的发病机制极其复杂，是多种因

8、素综合作用的结果，其中，肾小管上皮细胞