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ID:32354815
大小:3.40 MB
页数:31页
时间:2019-02-03
《klotho蛋白、caspase12蛋白在糖尿病肾病中的表达与意义》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、万方数据英文摘要Klothoproteinisprimarilyexpressedinrenalproximaltubules.Byimmunohistochemistrystaining,thepositivereactionofKlothowasidentifiedincytoplasm,characterizedbybrown—yellowishgranulesincytoplasm.Therewaspositivebasalexpressionintherenalproximaltubularepithelialcellsinthecontrolgro
2、up.TheexpressionofKlothoinDNgroupweresignificantlylowerthanthatofcontrolgroup(P<0.05).Caspase一12proteinisprimarilyexpressedinrenalglomeruliandtubules.Byimmunohistochemistry,thepositivereactionofcaspase-12proteinwasidentifiedincellnucleusand/orcytoplasm,representedbybrown—yellowishgr
3、anulesinthecytoplasmand/ornuclear.Therewaslightpositivebasalexpressioninthecontrolgroup.IntheDNgroup,thenumbersofpositive--expressedcellsandtheexpressionintensityofCaspase--12inrenaltissueweresignificantlyhigherthanthatofcontrolgroup衅0.01,氏O.05).Accordingtothecorrelationanalysis,the
4、reisanegativecorrelationbetweentheexpressionofKlothoandcaspase-12(F一0.643,P<0.05)inratswithDN.4TheapoptosisoftubularepithelialcellsbyTUNELstainingByTUNELstaining,theapoptoticcellswereidentifiedbybrown-yellowishgranulesincellnucleus.Theapoptoticcellswerecountedtocalculatetheapoptotic
5、index.TheapoptoticindexoftubularepithelialcellsinDNgroup(34.64-5.2)wassignificantlyhigherthanthatofcontrolgroup(1.6土1.2)仔k0.01).Conclusion:IthasshownthattheexpressionofKlothoproteinWasinhibitedandtheexpressionofCaspase一12proteinwasincreasedinrenaltubulesofratswithtype2diabetes.There
6、isanegativecorrelationbetweentheexpressionofKlothoandcaspase-12inratswithDN.Meanwhile,theapoptosisinrenaltubulescellsincreased.ItisproposedthatapoptosismediatedbyERstressmightbeinvolvedinthepathogenesisanddevelopmentofDN.Keywords:Type2diabetesmellitus,diabeticnephropathy,Klotho,Casp
7、ase一12,renaltubularepithelialcell,apoptosis7万方数据研究论文Klotho蛋白、Caspasel2蛋白在糖尿病肾病中的表达及意义前言糖尿病已成为全球性疾病,在世界人口死亡原因中位居第五位。我国的糖尿病患病率在10年期间增长了4倍以上,已经攀升到了将近10%,成为世界上发病最多的国家。2009年国际糖尿病联盟公布数据显示全世界已有2.85亿糖尿病患者,预计2030年达到4.35亿。糖尿病肾病是糖尿病最常见的并发症之一,愈来愈威胁着糖尿病患者的生存,成为终末期肾功能衰竭的主要原因之一。糖尿病肾病的发病机制极其复杂,是多种因
8、素综合作用的结果,其中,肾小管上皮细胞
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