klotho通过调控fgf2信号通路抑制肾间质纤维化的作用研究

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1、缩略语表英文缩写英文全称中文全称ARFAcuterenalfailure急性肾功能衰竭α-SMAα-smoothmuscleactin平滑肌肌动蛋白αC3Complementfactor3补体C3CCK-8Cellcountingkit-8细胞计数试剂盒CKDChronickidneydisease慢性肾脏疾病CRFChronicrenalfailure慢性肾功能衰竭DAB3’3-diaminobenizine二氨基联苯胺DAPI4,6-diamidino-2-phenylindole4,6-二脒基-2-苯基吲哚ddH2ODeo

2、ubledistilledwater双蒸水DMEMDulbecco’smodifiedeagle’smediumDMEM培养液DMSODimethylsulfoxide二甲基亚砜DNADeoxyribonucleicacid脱氧核糖核酸ECMExtracellularmatrix细胞外基质EDTAEthylenediaminetetraaceticacid乙二胺四乙酸ERKExtracellularregulatedprotein细胞外调节蛋白激酶KinasesFBSFetalbovineserum胎牛血清FGF2Basicfi

3、broblastgrowthfactor-2碱性成纤维细胞生长因子2FGF23Basicfibroblastgrowthfactor-23碱性成纤维细胞生长因子23FGFRFibroblastgrowthfactorreceptor成纤维细胞生长因子受体FITCFluoresceinisothiocyanate异硫氰酸荧光素FRS2αFibroblastgrowthfactorreceptor成纤维细胞生长因子受体底物2αsubstrate2αFSP-1Fibroblastspecificprotein1成纤维细胞特异蛋白1HE

4、Hematoxylin-eosin苏木精-伊红IgGImmunoglobulinG免疫球蛋白GNRK-49Fnormalratkidney大鼠正常肾成纤维细胞ODOpticaldensity光密度值PCRPolymeraseChainReaction聚合酶链式反应PBSPhosphatebalancedsolution磷酸盐缓冲液RNARibonucleicacid核糖核酸ScrSerumcreatinine血清肌酸酐TGF-β1Transforminggrowthfactor-β1转化生长因子β1UUOUnilateralur

5、eteralobstruction单侧输尿管梗阻万方数据Klothoinhibitsrenaltubulointerstitialfibrosisbyregulatingbasicfibroblastgrowthfactor-2signalingAbstractRenaltubulointerstitialfibrosisisacommonpathwayinchronickidneydisease(CKD)thatleadstoend-stagerenalfailure.Itstwoprominentpathologicalch

6、aracteristicsareexcessivedepositionofextracellularmatrix(ECM)componentsandaccumulationofinterstitialfibroblasts.PreviousstudiessuggestthatthemyofibroblastdifferentiatedfromfibroblastisthemainsourceofECMandthattubuloepithelialcellremodelingcanresultinamyofibroblastphe

7、notypeandthesynthesisofmatrixproteins,suchastypeIandIIIcollagens.Thus,inhibitingfibroblastproliferationanddifferentiationandtubuloepithelialcellremodelingisanimportantwaytoreducethenumberofmyofibroblastsandECMsynthesis,andthestudiesontheunderlyingmechanismscanprovide

8、anovelstrategyandtargetforpreventionandtreatmentofrenalinterstitialfibrosis.Basicfibroblastgrowthfactor-2(FGF2)isinvolvedinprolifer