骨化三醇对uuo模型大鼠肾间质纤维化抑制作用与其对内皮细胞参与纤维化调控作用的-研究

《骨化三醇对uuo模型大鼠肾间质纤维化抑制作用与其对内皮细胞参与纤维化调控作用的-研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、英文摘要1．5The1，3，5daysgroupexpressionofVEGF,VEGFR一2，HIF-lotbywesternblot．2Cytologytestofcalcitriolonendothelialcellsinvolvedinthemechanismoffibrosis．2．1Endothelialcellsculture：Eahy926，CulturedunderhighglucoseDMEMmediumcontaining10％fetalbovineserumat37。C，5％ofC02，satura

2、tedhumidity，toEahy926cellsgrowthmonolayerspare．Eahy926cellweredividedintothefollowinggroups；@Cocl2controlgroup(cobaltchloride)，@Blankcontrolgroup(EqualvolumeofD·Hank’Ssolution)，③Highdosegroupofcalcitriol(finalconcentration10一mol／L)，@Mediumdosegroupofcalcitriol(fina

3、lconcentration10一mol／L)，@Lowdosegroupofcalcitriol(finalconcentration10。10mol／L)．2．2ProliferationeffectofcalcitriolinThecobaltchloridestimulationEahy926cellsmeasuredbyMTT．2．3TheexpressionofVEGFlevelsineachgroupbyELISA．2．4TheeffectofcalcitriolinEahy926celldeterminedb

4、yRT-PCRandwesternblot．DetectingtherelativecontentofEahy926cellsmRNAonVEGF,VEGFR-2，HIF-ls，ICAM-1andproteinonVEGFR-2，HIF-ls，ICAM-1．2．5Endothelialcellapoptosisratedetectedbyflowcytometryineachexperimentalgroup．3Theenumerationdatawasrecordedas?C土s．Results：1Effectofcalc

5、itriolonpathologicalchangesinratrenalinterstitialfibrosis。AfterUUO，thetumafactionandatrophyinrenalparenchymaofobstructed，kidneysweremeasuredobviously．Shamoperatedgroupdidnotchangesignificantly．HEstainingobservedUUOgroupfoundtubulointerstitialinflammatorycellsincrea

6、sed，Varyingdegreesoftubularcelldegeneration，atrophyandnecrosis．TheHighdosecalcitriol，lowdosecalcitriol，thebenazeprilgroupKidneyfibrosismilderUUOgroupatthesamepointintime．SiriusscarletstainingobservedUUOgroupproductcollagenincrease，decreaseineachdrugtreatmentgroupco

7、llagen，Nosignificantchangesin6英文摘要——————————————————————————————————————————一一一shamoperatedgroup．2Calcitriolchangeson7，10，14daysserumTNF．aconcentrations．TNF-0【concentrationsofeachdrugtreatmentgroupsandUUOgroupthantheshamoperatedgroupincreased妒

8、ction，meincreasingexpressionofTNF一0【．Hi曲andlowdoseofcalcitriolandbenazeprilcanreducetheUUOratserumconcentrationofTNF．ct(P<0．05、1．3Effectofcalcitr