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1、#%S*S&NB%B*ZtBPCR&jJ!g:gProtocolofthequalitativepolymerasechainreactionfordetectinggeneticallymodifiedplantcomponentsinfood~3n~~f+t:~)1m~kaa*k@m~1milfiljt%*h%m%&~nmfl:.asrsssmxFa+le.mmi%H#&@t~~R&~@)~#t5%~4BH;f.;4%~t%TmtBfiRB2P;Z~B&B~~%3iWf%ABRf@!HBBAt+;9~%E*eRR+%
2、f:~tgl5lMFf]R~1~~~zb:~H~*~~.GB/T6682j)$i%&SH7k%#j#IBtk&(eqvIS03696:1987)SNIT1193J#B@!&%&3Z&*BjkSNIT1194M@B#~#I%&~&~@B!!~#~#~!#-)~%SN/T1195hEi+$$SL!4&3)Z?4I'CRUB!!YJ'&SN/T119639%PCR%%!!h?&SN/T1197?h%RR&B3&3)2#PCR3$ildhk&SN/T1199#l%+R&B3&3)PCR&@I%-&SN/T1204t$~B#%fi~S
3、C~&~%3&~r3t3)Td'U~%XPCR%&%t%?f?&TPl@%%&HT*&%*3.1PCRIpolymerasechainreaction,m%PCR.3.2DNA1de~x~ribonucleicacid,%%@@j&@.3.3dNTP:deoxyribonucleosidetriphosphate,8#$t%3?@@.3.4dATPldeoxyadenosinetr~phosphate~Llk!%IlTT@@.3.5dCTP:deoxycytidinetriph~sphate,a#SmBE-R@~3.6dGT
4、PIdeoxyguanosinetriphosphate,R#$€~~7@@3.7dUTPdeox~ur~dinetriphosphate,Jl$!$G$e3.aft@3.8bpbasepair,@SN.3.9TagBrDNAJ@*m.3.10'I'rls,tris(hydroxymethyl)amlnoniethanc,!-:(%!pg)&,%V%$.,3.11TE:T~I~-CI.EDTA$~!~~~$..3.12I.ectinl&44&l%~.3.13ZEINi#&%B$#E$.3.14~~3-~~~case1~hos
5、~hoenil~~ruvate-carboxylahc,b#@~~~aj~@;)fat.(t:~.'3.15~atat~n,~##&ZE%Z?lAPalatinBH#&jttlR%q%BHli4%%fl:e3.16tRNA~eu~&kQdWflW#4f!il?il.3.1718srRNAl&&%@118sRNA%a.3.18CaMV35S135Spromoterfromcauliflowermosaicvirus,?f€aT8@%%lJt$i%l8J35SBaT.3.19NOS,terminatorofnopalinesyn
6、thasegene,MRB~-&lilt~~~~k~.3.20FMV35S135Spromoterfromamodifiedfigwortmosaicvirus(caulimovirusgroup),g8ZR-tR*35saa~.3.21NPTII:neomycin-3'-phoephotrmsforasegene,#$#-3'-@@R@BS88.3.22BAR:phosphinothricinacetyltransferasegenefromBacillusamyloliquefacions,?#@TfimBacillus
7、amyloliquefacisnsVTj#(Ptl%%@E#SBj.3,23PATIphosphinothricinacetyltransferasegene,9TM2E?Rl$~&B.3.24COXIglyphosateoxidoreductaaegene,W€FM~gtEHll#ftlE~.3.25CryIA(b)lasyntheticgeneencodesthefiret648aminoacids,insecticidal-activeftruncatedproductidenticaltothatofcryIA(b)
8、geneofBacillusthuringiensissubsp,%2$?$84HB%&isI3crylA(b)SB.3.26Cry3AlB,thuringiensissubsp,Tenebrionis(B,t,t,)strainQI256-82B&@sfi,#k@*#bjQ&Il~*