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1、Protocolofthepolymerasechainreactionfordetectinggeneticallymodifiedcomponentsinpotatoes*&@BB74%S+H%SBB3)HBtkPCRU1k?kO;4&3;B7@q9@ap"a$$(NEWLEAPPOTATOLINESBT-06,ATBT04-06,ATBT04-31,ATBT04-36ANDSPBT02-05;(NEWLEAPPLUSPOTATOLINESPBMT21-129,PBMT21-350ANDPBMT22-8
2、2i(NEWLEAF@Y)POTATOLINESRBMT15-101,SEMT15-02ANDSEMT15;15%&HH%SB4@S&%-.tf[&~4S0iFA,4SiF~@8HUStle7;PJ$tES@ZX(S@B)3H;f;46@.3.1transgene~#Hr4W6W~H~sff%TJ%fi!i~HHrJ%DNABFU*a3&H%APR,@XBB+a?74&9RE%@lWIR.?f)8JiHp"a$$f&E.3.2ff&*@$EBipolymerasechainreaction,PCR#i&iS
3、BFFPJ5'C8!i@B!Etk&%##~~DNA@&WFH@i3BHlZG%#7;,@@i#&iJYPJi2itHH&51B3)%1-4&&DNAH%Pk#GH-&X+E89'J84Bkmi#4:%+,#&S?!EDNASeIH+H7;RDlWI%SB%~@(dNTP)%liii@,@5I~%U@j~,#G6WfiP~#~~k~I#Ij$ia-#w,@arMHsm%~vALm#%r*o3.3P%ia3.3.1Pata:4%%lWIMfRl!kDNA31%,~Hl!H%€3%4@%?ASafllEHElPat
4、atinSB#@.3.3.2CaMV35S:promoterfromcauliflomermosaicvirus,^&^&^$$@35sg33.3.3.3NPT-1:neomycinphosphotransferase-fl,~~~-3'-@@%~m3.3.4N0S:nopalinesynthaseterrninator,@lflB%6Bm3'%3%k3.3.3.5PVYcp:potatoYviruscoatprotein,4+$%YYS9ESEPQ.3.3.6cry3A:-+AB.thuringiensiss
5、ubsp,Tcnebrionis(B.t.t.strainBI256-82Hgh.j&/FcgEIW8%&%tk,ft$$EEJ~jtk~%~+4;C~~44ST&(Coloradopotatobeetlelarvae).13.3.7CP4EPSPS:5-enolpyrulshikimate3-phosphatesynthase5-enolpyrulshikimate3-phosphatesynthase,*aT$!fi@Agrobacteriumsp.StrainCP4H5-@*@-3-@@g&j@3.3
6、.8PLRVrep:potatoleafrollvirus,$#S%W#3SSBFFPJ3.3.9E93':the3'non-translatedregionofthepearibulosel,5-bisphosphatecarboxylasesmallsubunit(rbcS)E9gene.,SRBTWiZHBM#-l,5-I.@@#t%j@E9SBi/JEi&3'%lJ?PJ3.3.10ArabSSU1A:arabidopsisthalianaribulosel'5-bisphosphatecarboxy
7、lase(Rubisco)smallsubunitats1Apromoter,arabidopsisthaliana~&~#-1,5-1@@#t%B~JE1AmB37b3.3.3.11aad:(residesoutsideT-DNA)?ZffBI3fin7rfrSi@%SAjeSa%E(S)ffjk%j@HSBiO3.3.12oriV:(residesoutsideT-DNA)--+@$$!REA~grobacteriumHRK21.3kbMBBJEM03.3.13ori322t(residesoutside
8、T-DNA)-+%iGpBR322BBm1.8kbH#&,#rfr&-$g$djEj&%l4%4$%LAbom.3.3.14FMV35s:apromotorderivedfromfigwortmosaicvirus(FMV),g@zw$$S35sa?.I5.2q*mW##5.2.1~~ISZ.&ISB.&aR.;(s[~HS##O~~~-~&~~es~s.*s~.*s#,~$+#a