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ID:32341580
大小:5.20 MB
页数:52页
时间:2019-02-03
《rnai沉默pttg对大鼠垂体腺瘤细胞cd147、mmp-9表达的影响》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、TheexpressionofCDl47andMMP--9inratpituitarytumorcellsbyRNAitargetingPTTGBackgroundSpecialityPh.MSupervisorSurgery(Neurosurgery)FanShengqiangProf.XuGuangmingABSTRACTPituitarytumortransforminggene,alsoknownasanoncogene,wasdiscoveredinratpituitarytumorcellsbyPel.etalin1997.Inthefurther
2、research,wefoundthatP1vrGWaShighlyexpressedinvarioustumorsincludingcolon,ovary,testis,lung,andbreaSt.PTTGplayedallimportantroleininhibitingseparationofsisterchromatidandtherepairingofDNA.AnoverexpressionofPTTGalsoinducedthetumorangiogenesis,aggressiveandmetastatic.CD147,knownasExtra
3、cellularmatrixmetalloproteinasesinducer(EMMPRIN),isamemberoftheirnrnunoglobulinsuperfamily.CD147isatransmembraneglycoproteinwithtWOirnrnunoglobulin..1ikedomainsandinvolvesinvariousimportantsignaltransductionofcells.Inthestudyoftumoraggressiveandmetastatic,wefoundthathighlyexpression
4、ofCD147hasallintimaterelationshipswithtumorprogression.CD147promotethetunlorcellssecretionmatrixmetalloproteinases,andenhancetheaggressiveandmetastaticoftumorcells.MatrixMetalloproteinases.9(MMP.9)isamemberofmatrixmetalloproteinaseswhichplaysanimportantroleintissuedestructionandinfl
5、ammationthroughdegradationofmatrixproteinsandproteolyticactivationofcytokines.expressionofMMP一9intumorwaSmorethanthenomaltissual,atthesarnetime,MMP-9participatedintheprocessoftumoraggressive.PTTG,CD147and1山东大学硕士学位论文MMP-9allplayedimportantrolesintumorigenesis,theexperimentthroughRNAi
6、nterferencetargetingPTTGofratpituitarytumorcells,todetecttheexpressionsofCD147andMMP一9whichwerecorrelationedwiththetumoraggressive.ToresearchtherelationshipsofPTTG,CD147andMMP-9insignaltransductionoftumorcells.Forfurtherstudying,theinvasionandapoptosisofpituitarytumorcellsbyshRNAtar
7、getingPTTGIthadmorecleardefinitionaboutthefunctionofPTTGintumorigenesis.Theexperimentdividedintotwoparts.ITheexpressionofCDl47andMMP一9inratpituitarytumorcellsbyRNAitargetingPTTGobjectTostudytheinfluencesofexpressionsofCD147andMMP.9inGH3ratpituitarytumorcellsbyRNAitargetingPTTGMethod
8、sGH3cellswereculn】r
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