rnai沉默pttg对大鼠垂体腺瘤细胞cd147、mmp-9表达的影响

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1、TheexpressionofCDl47andMMP--9inratpituitarytumorcellsbyRNAitargetingPTTGBackgroundSpecialityPh．MSupervisorSurgery(Neurosurgery)FanShengqiangProf．XuGuangmingABSTRACTPituitarytumortransforminggene，alsoknownasanoncogene，wasdiscoveredinratpituitarytumorcellsbyPel．etalin1997．Inthefurther

2、research，wefoundthatP1vrGWaShighlyexpressedinvarioustumorsincludingcolon，ovary，testis，lung，andbreaSt．PTTGplayedallimportantroleininhibitingseparationofsisterchromatidandtherepairingofDNA．AnoverexpressionofPTTGalsoinducedthetumorangiogenesis，aggressiveandmetastatic．CD147，knownasExtra

3、cellularmatrixmetalloproteinasesinducer(EMMPRIN)，isamemberoftheirnrnunoglobulinsuperfamily．CD147isatransmembraneglycoproteinwithtWOirnrnunoglobulin．．1ikedomainsandinvolvesinvariousimportantsignaltransductionofcells．Inthestudyoftumoraggressiveandmetastatic，wefoundthathighlyexpression

4、ofCD147hasallintimaterelationshipswithtumorprogression．CD147promotethetunlorcellssecretionmatrixmetalloproteinases，andenhancetheaggressiveandmetastaticoftumorcells．MatrixMetalloproteinases．9(MMP．9)isamemberofmatrixmetalloproteinaseswhichplaysanimportantroleintissuedestructionandinfl

5、ammationthroughdegradationofmatrixproteinsandproteolyticactivationofcytokines．expressionofMMP一9intumorwaSmorethanthenomaltissual，atthesarnetime，MMP-9participatedintheprocessoftumoraggressive．PTTG，CD147and1山东大学硕士学位论文MMP-9allplayedimportantrolesintumorigenesis，theexperimentthroughRNAi

6、nterferencetargetingPTTGofratpituitarytumorcells，todetecttheexpressionsofCD147andMMP一9whichwerecorrelationedwiththetumoraggressive．ToresearchtherelationshipsofPTTG，CD147andMMP-9insignaltransductionoftumorcells．Forfurtherstudying，theinvasionandapoptosisofpituitarytumorcellsbyshRNAtar

7、getingPTTGIthadmorecleardefinitionaboutthefunctionofPTTGintumorigenesis．Theexperimentdividedintotwoparts．ITheexpressionofCDl47andMMP一9inratpituitarytumorcellsbyRNAitargetingPTTGobjectTostudytheinfluencesofexpressionsofCD147andMMP．9inGH3ratpituitarytumorcellsbyRNAitargetingPTTGMethod

8、sGH3cellswereculn】r