抑肽酶对凝血酶诱导的体外星形胶质细胞凋亡性损伤的保护作用的研究

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1、第三军医大学硕士学位论文英文缩写词表英文简称英文全称中文全称AAprotinin抑肽酶AOAcridineOrange吖啶橙ASTAstrocyte星形胶质细胞BSABovineserumalbumin牛血清白蛋白dDay天DMEMDulbecco’smodifiedeaglemedium改良的基础培养基DMSODimethylsulphoxide二甲基亚砜EBEthidiumBromide溴化乙锭FBSFetalbovineserum胎牛血清FCMFlowcytometry流式细胞仪检测术FITCFluoresceinsothioyanate异硫氰酸荧

2、光素GFAPGlialfibrillaryacidicprotein胶质原纤维酸性蛋白hHour小时ICCImmunocytochemistry免疫细胞化学ICHIntracerebralhemorrhage脑出血IEGImmediateearlygene即刻早期基因KDaKiloDalton千道尔顿KIUKallikreininhibitorunit激肽释放酶抑制单位LDHLactatedehydrogenase乳酸脱氢酶LMPLowmelting-pointgelose低融点琼脂糖minMinute分钟NMPNormalmelting-pointge

3、lose正常融点琼脂糖PAR1Protease-activatedreceptor-1蛋白酶激活受体-1PAR2Protease-activatedreceptor-2蛋白酶激活受体-21第三军医大学硕士学位论文PAR3Protease-activatedreceptor-3蛋白酶激活受体-3PAR4Protease-activatedreceptor-4蛋白酶激活受体-4PBSPhosphatebufferedsaline磷酸盐缓冲液PIPropidiumiodide碘化丙啶PKKProteinkinaseK蛋白激酶KrpmRoundperminute

4、每分钟转数sSecond秒SABCStreptavidinbiotincomplex链亲和素-生物素复合物法TBETris/borateelectrophoresisTris/硼酸电泳缓冲液TEMTransmissionelectronmicroscopy透射电镜TRThrombinreceptor凝血酶受体UUnit单位2第三军医大学硕士学位论文ProtectiveeffectofaprotininonapoptoticinjuryofastrocytesinducedbythrombininvitroAbstractObjective:Clinica

5、llybraincellinjuryafterintracerebralhemorrhage(ICH)alwayscausesdysfunctionordeathonsuchpatients.StudieshadidentifiedthatthemassofthrombingeneratedintheprocessofbloodclottingmaycontributetothebraincellinjuryafterICH.Thrombin,akeyenzymeinthe“waterfallreaction”ofbloodclotting,isalso

6、knownasthemultifunctionserineproteaseenzymeinotherfield.Therearegreatdealsofthrombin-activatedreceptors-protease-activatedreceptors(PARs)onthebrainastrocytes.ThrombinmightparticipateintheapoptoticinjuryofastrocytesthroughPARs.Aprotinin,anaturalserineproteaseinhibitorderivedfrombo

7、vinelung,hadbeenreportedthatitcouldaccommodatethecellreactionswhenPARswereactivatedinourpreviousstudy.However,itremainsunclearwhetheraprotininhadprotectivefunctionstotheinjuryofastrocytescorrelatedwiththrombin.Inthisstudy,Astrocyteswereculturedprimarilytostudytheactionofthrombini

8、nvitro.Differentpreconditiondosageswerec