fxr对apom表达的影响与其机制的初步探讨

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2、文缩写apoMASp-galBSEPCACDCACYP7AlDBDcDNAFXRFXREHDLCHLBDLucPIJPRT．PCRRXRSHPLDLWBAAAmpAPSDEPCDAB英文缩写一览表英文全称ApolipoproteinMatherosclerosisbeta—galactosidasebilesaltexportpumpcholicacidchenodexycholicacidcholesterol7a—hydroxylaseDNA-bindingdomaincomplementaryD

3、NAfamesoidXreceptorFXRresponseelementhigh—densitylipoproteinCholesterolligand—bindingdomainluciferasephospholipidtransferproteinreversetranscription-polymerasechainreactionretinoidXreceptorsmallheterodimerpartnerlowdensitylipoprotein阮stemblotAcrylamideA

4、mpicillinAmmoniumpersulfateDiethylPyrocarbonateDiaminobenzidine中文全称载脂蛋白M动脉粥样硬化p一半乳糖苷酶胆盐输出泵胆酸鹅脱氧胆酸胆固醇70【羟化酶DNA结合域互补DNA法尼酯X受体FXR反应元件高密度脂蛋白胆固醇配体结合域荧光素酶磷脂转运蛋白逆转录．聚合酶链反应视黄醛X受体小异二聚体伴侣分子低密度脂蛋白免疫印迹实验丙烯酰胺氨苄青霉素过硫酸铵二乙基焦磷酰胺二氨基联苯胺第二军医大学硕+学位论文DMSOSDSPAGETween．20TrisT

5、EMEDTGTCDimethylsulfoxide二甲基亚砜Sodiumdodecylsulphate十二烷基硫酸钠Polyacrylamidegelelectrophoresis聚丙烯酰胺凝胶电泳Polyoxyethylene-20一SorbitanMonolautate吐温．20Tris(hydroxymethyl)aminomethane三羟甲基氨基甲烷N，N，N，N-tetramethylethylenediamine四甲基乙二胺triglyceride甘油三酯Fotalcholesterol

6、总胆固醇2toSolvetheproblem，wewillfurtherclarifytheroleofFXRandapoMintheoccu盯enceanddevelopmentofAS，targetpreventandcurethenfinallyprovideanewscientificevidenceforfindinganewtheAS．3第二军医人学硕十学位论文objective：ToinvestigateFXReffectstoapoMexpression，andthepossiblem

7、echanismofFXR．Methodology：(1)SelecthepatomacellHepG2andfetallivercellL02asthecellmodel．Test1treatedthecellmodelbyCDCAfor24h，thendetectthechangesofapoMexpressionbyusingRT-PCR；test2，treatedbyCDCAfor12h，thentreatedbyinhibitorGuggulsteronesfor24h，detectthec

8、hangesofapoMexpressionbyusingRT-PCR．(2)SelecthepatomacellHepG2ascellmodel，detectthedifferencesofapoMexpressionbyusingWesternBlot．(3)SelectC57BL／6miceasanimalmodel，feedthemwithCDCA，anddetectthechangesofapoMexpressionbyRT-PCR，andth