迷迭香酸对放射dna损伤的防护作用与其机制的初步分析

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1、迷迭香酸对放射致DNA损伤的防护作用及其机制的初步研究者有密切的关系。结论：迷迭香酸在体内、外照射模型上均能剂量依赖地降低小剂量Y线照射引起的细胞微核发生率；照前给药有效，照后给药效果不明显；适当延长给药时间可增强迷迭香酸的体内疗效。初步机制研究提示，迷迭香酸可能通过降低细胞内ROS生成和增加细胞I内GSH含量达到减轻放射致DNA损伤的作用。这些结果提示，迷迭香酸有望开发成高效、低毒的新型DNA放射损伤防护药物。关键词：迷迭香酸；60Co丫射线；微核；ROS：GSH作者：王淑伟指导老师：从玉文appear．Owingt

2、otheirnon-orlower-toxicityatpharmacologicaldosesthansyntheticradioprotectants，generousantioxidantssourcefromnaturalproductsweremoreandmorepopulartobethoughtasaradioprotectant．objective：Toexploretheeffectofrosmarinicacid，asmall—moleculenaturalproduct：rosmarinicac

3、id，hasbee．nreportedasanantioxidantonmicronucleusformationrateofcellsirradiatedwithcobalt-60T-raysinvitroandinvivo，andtoinvestigatethepreliminarymechanismofaction．Methods：V79cellsweretreatedwithrosmarinicacidbeforeirradiated4．0Gycobalt-60),-rays，andthemicronucleu

4、srateinthecellsWascountedtoexplorerosmarinicacidasaradioprotectant；C57BL／6Jmicewereirradiatedwith2Gyafterwithadministrationrosmarinicacid，andthemicronucleusrateinpolychromaticerythrocytesofbonemarrowcellsWascountedtoexplorerosmarinicacidasaradioprotectant；Thecon

5、tentofROSincellsandtheleverofglutathione(GSH)inbonemarrowcellsweremessuredbyVICTORTMX5．Results：Firstly，themicronucleusrateofV79cellsWascountedtoprove英文摘要整堡重墼翌垫塾鍪旦翌垒垄堡箜堕芝堡旦墨苎塾型塑塑垄堡壅-__--_●I___-__I__-l●__--●-_--______-__-__--I-●_-_____——-—__-_-●___●_————————————一—

6、—一cellsw弱counted．Weconfirmedthattheexperimentused2cyT-rayastheanimalmodelofradiationinjuryinvivo．Radiation-inducedmicronucleusrateinpolychromatieerythrocytesofbonemarrowcellscouldbereduceddependentlybywithadministrationrosmariIlicacid．Itwasmoreobviousbefore2GyT-

7、raywithadministrationrosmarinicacidthanafter2Gyp豫ywithadministrationmsmarinicacid，anditcouldincreaseefficacytoappropriatelyprolongactiontimeofrosmarinicacid．MicereceivedadailyoralofrosmarinicacidlOOmg／kgfor7consecutivedays，andbonemarrowmicronueleusrate(6．50％o)co

8、uldreducetOcomparewithradiation-induced(23．65％0)，．andit’SlowerthanvitaminC(14．51％0)．What7Smore，primarystudyonthemechanismofrosmarinicacidagainstgammarayinducedDNAdama