菠萝半纤维素多糖提取、纯化和免疫活性的研究

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1、摘要为了探求加工废弃物菠萝皮渣新的利用途径，雄文以果汁加工厂的菠萝皮渣为材料，对菠萝毕终维素多耱进蟹了提取，臻耀苯酪-虢敦法秘＆l纽．酶滚瓣萁慧耱含量窝爨鱼鸯鬣避莓了测定。菠萝半纤维索糨多糖PAP4和PAP5的糖含量分别为55．13和60．05％，蛋白禽艇分别为4．75和2．08％。袋罔离予交换和凝胶过滤屡糖技术对半纤维粢多糖进褥丁癸离纯纯，褥辩瓣个纯度溜一靛酸梅多糖缀箭PAP4一致和PAP5，珏}。荣掰HPI．C技术对箕分子蛩敝单糖组成和含量鲑}行了测定，两个海糖的分予量均犬予2000KD。且都是由木糖和阿拉伯糖

2、两穆鼙糖夔襄，萁中PAP4-Ii孛嚣种耱熬禽鼙努烈蠹涟缆鞠39．32：＆g／g，PAPS-ItP孛两种耱的含髓分别为68．69和24．53mg／g。对PAP4．II和PAP5-IIP进行了紫外、红外和核磁燕振捡溅，慰冀鳝稳髂惠有了裙步静了解。采耀棼菸器B港巴馏整增蓬实验，对菠爹半绎缆索租多糖组分PAP4和PAP5以及两个纯度均～组分PAP4-II和PAPS．IIP的免疫活性进行丁研究。四个多耱缎分，对弘B潺巴缨魏增殪均表现出摊越终雳。在实验用榉浓度下，除PAP4-II对枣鬣t淋琶缀胞增整静抑制律用与样品浓度璺弱疹增

3、长关系井，其余的均存在一个界限用样浓度点。当样品浓度小于该用榉浓度时，熊抑制作用随样鼬浓度的增加丽增强，但当槽县浓魔大予该震搭浓度煮辩，箕簿濑露露薮掰会下辩。实验串避发褒，在毵样品浓度簿，PAP5对B淋巴细胞的增殖有～定的促进作用，但作用并不很强。关键词：菠萝半纤维素多糟纯化免疫活性AbstractTofindanewwayoftheutilizafionofpineapplepomace，thehemiceUuloscpolysaccharidewasextractedfromthepineapplepomace

4、discardedbysyrupfactor,thecontentsoftotalsugarandproteinweredeterminedusingPenol—VitriolmethodandFolin*Pen01．Method．Thesugarcontent。fthepineapplehemicellulosepolysaecharidePAP4andPAP5wele55．13and60．05％respeetively，andtheproteincontentofthemwere4．75and2．08％．The

5、pineapplehemicelhlosepolysaecharidewaspurifiedusingionchangechromatographyandsizeexclusionchromatography,andtwoacidpolysaceharidesPAP4一IlandPAP5一IIP∽regained．ThemolecularweightofthemwasdeterminedbyHPLC,monosaechaddecompositionandcontentwerealsodeterminedbyHPLC

6、．Themolecularweightofthetwoacidpolysacchaddeswerebothmorethan2000KD，andtheyarebothcomposedofxyloseandarabinose，thecontentsofxyloseandarabinoseofPAP4·IIare54。02and39．32rag／g,andthecontentsofxyloseandarabinosoofPAP5一嚣Pwelx：68。69and24．53mg／g．TheUVscan，IRscanandNM

7、RSCAnWeredone，andsomeprimaryinformationaboutthestructureofthepolysaccharidesweregainedfromthem，TheimmunobiologiealactivityofthetworudehemicellulusepolysaccharidesPAP4andPAP5andthetwopurifiedacidpolysaccharidesPAP4-IIandPAP5一IIPweI_estudiedbyTandBlymphcellsexpe

8、riment,andtheyallrestrainedtheincreaseofTandBlyre#cells．Atthemustercontentoftheexperiment，theretrainingeffectofpApa,-IItothemultiplicationofTlymphcellsincreasedfollowingwiththeincr