灰黄霉素产生菌fs80的诱变选育的的研究

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1、福建师范大学张剑清硕士学位论文少，发酵产灰黄霉素效价比出发菌株FS80．1及突变株LDl00．1高。关键词：诱变：激光；灰黄霉素：效价；发酵；IIAbstractGriseofulvinproducingstrainPenicilliumpatulumFS80-1wastakenastheoriginalstrain,combinedtreatmentofUVandLiCl，thehi曲-yieldmutantGM120·43wasobtainedafterthreegenerationsofselection,whichproducinggrise

2、ofulvincapacitycouldbe89496I-tg／gusingdiazoniumsaltcolordetection,comparedwiththeoriginalstrainFSS0-1whichincreasedby80．04％．MutantstrainGMl20-43geneticstabilitywaswell．Andthen，mutantGM120-43wastakenastheoriginalstrain,mutantLDI00-1andC1448—1wererespectiveobtainedbysemiconductor

3、andC02laser．Aftergeneticstabilitytest，thetiterofmutantLDI00-1producinggriseofulvinWas19766pedg,improved22．58％thantheoriginalstrainGMl20-43．ThemutantC1448·lobtainedbytheC02lasertestedbygeneticstability,whichproductionofgriseofulvinWaslowerthanstrainGMl20-43．Thesolidculturecondit

4、iomofmutantLDl00-1wereresearched，theresultsshowthat：theoptimumcarbonSOUrC2substrateWasWanxianMi；theoptimumnitrogenWasNaN01；thesuitableradioofmediumsolidtowaterWas1：O．75；thesuitableprecursorschlorideforthesynthesisofgriseofulvinwassingleaddingNaCI，andtheconcentrationofchlorideWa

5、sO．188mol／LWasmoreappropriate．；Seedswereinoculated、)lri吐1liquid，thesuitableageofinoculatedliquidseedwas44h'inoculumwas10％．Afteroptimizationofcultureconditions，thetiterofgriseofulvinproductedbystrainLDl00一lWas162233“g／g，whichincreasedby39．16％beforeoptimization．Tllecultureriscesp

6、roeofstrainoriginalstrainFS80—1andmutantGMl20—43，LD100-1wereextractedwithethan01．ThecontentofgriseofulvininextractsWasdetectedbyHPLC，comparedwimthedifferencedetectionmethodsofHPLCanddiazoniumsaltcolor,theresultsshowedthat：thecapacityofstrainFS80·1，GMl20·43andLDl00一lproducinggri

7、seofulvinwere43511rtg／g．91858Itg／gand105423p∥g，whichwerelowerthanthediazoniumsaltcolormethod．ExtractthegenomicDNAofmutantGMl20-43，LDl00-1andoriginalstrainFS80-1．，andtakegenomicDNAastemplate，using18SuniversalprimersforPCRamplification，sequencingtheamplified18SrDNAandcomparedeach

8、other．TheresultsshowedthatthedifferencesbetweenmutantG