刚果嗜皮菌选择性分离方法的的研究与盘羊分离株的分离鉴定

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1、AbstractDermatophiluscongolensisisamemberofActinomycetales,Dermatophilaceae,Dermatophilus.Causedthediseasesofanacuteorchroniconavarietyofanimalandhumanbytheskincontanct.Withsuperficialexudativepustulerashornodulesasthemainclinicalfeatures.Theinfectionmaycauseadeclineinproductivityandresis

2、tanceofdisease,infectiononSheepcanleadtoreducethequalityofwoolandleather,OIEliststhediseaseinBclass.BecausethecharacteristicsofthegrowthofDermatophiluscongolensisinsporesandhyphae.Dermatophiluscongolensisisolatedfromskinlesionsisoftendifficulttoobtainpureculturebacteria.Thereforetheestabl

3、ishmentofanefficientandeffectiveseparationoftheDermatophiluscongolensis.ToisolatedandidentificateofDermatophilusCongolensisofArgalisheep.Inthisstudy,purifiedandidentifiedsheepstrainsofDermatophiluscongolensisasmaterial,thedifferentpretreatmentmethodsandtheculturemediumwasusedfortheisolati

4、onofDermatophiluscongolensis.establishedaeffectmethodwasforlaboratoryexperiment.Resultsshowthat:1．Theskinmaterialwasdriedat28℃andsmashed,addedwithsterilewaterfor15days,standing.Addedwith6%yeastextractwasstirredat60℃for30minandscribbledonplatesat37℃for48h,toevaluatethecharacteristicsoftheD

5、ermatophilusCongolensis,37℃waterbathed1h,orplacedincandle15minisolatednobacteria;0.05%SDSandthe0.05%SDS6%proteinpeptoneisaddictedtotheactivationofthebacteria;ThebacteriaonGauze’mediumNo.1poorgrowth;LBagarisinferiortosheepbloodagar.2．ThebacteriacollectedfromArgalisheepwererelatedtotheDerma

6、tophilusCongolensis.thebacterialmorphology,cultivatingcharacteristics,indirectimmunohistochemistrywereperformedanddevisedapairofspecificprimersaccordingtothe16SrRNAsequenceofDermatophilusCongolensisGenBankpublishedforPCR.3．TheresultofDNArandomamplifiedpolymorphicDNA(RAPD)analysisshowedtha

7、ttwodifferenthostsoriginateDermatophilusCongolensishavedifferentgenome.Keywords:Dermatophiluscongolcnsis;isolationandidentification;RAPDII符号说明——英文缩略词表英文缩写英文全称中文名称TEMEDteramethylethylenediamineN,N,N’,N’-四甲基乙二胺Isopropyl-β-D-thiogalactopyrIPTG异丙基硫代-β-D-半乳糖苷anoside3,3-d