抗汉坦病毒单链抗体2fcκ2fprotamine截短体融合蛋白的基因构建、表达与功能的研究

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1、独创性声明秉承学校严谨的学风与优良的科学道德，本人声明所呈交的论文是我个人在导师指导下进行的研究工作及取得的研究成果。尽我所知，除了文中特别加以标注和致谢的地方外，论文中不包含其他人已经发表或撰写过的研究成果，不包含本人或他人已申请学位或其他用途使用过的成果。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明确的说明并表示了致谢。申请学位论文与资料若有不实之处，本人承担一切相关责任。论文作者签名：日期：保护知识产权声明本人完全了解第四军医大学有关保护知识产权的规定，即：研究生在校攻读学位期间论文工作的知识产权单位属第四军医大学。本人保证毕

2、业离校后，发表论文或使用论文工作成果时署名单位仍然为第四军医大学。学校可以公布论文的全部或部分内容（含电子版，保密内容除外），可以采用影印，缩印或其他复制手段保存论文。学校有权允许论文被查阅和借阅，并在校园网上提供论文内容的浏览和下载服务。论文作者签名：导师签名：日期：抗汉坦病毒单链抗体/Cκ/protamine截短体融合蛋白的基因构建、表达及功能研究研究生：陈锐学科专业：生物化学与分子生物学所在单位：第四军医大学生物化学与分子生物学教研室导师：杨安钢（教授）辅导教师：杨洁（讲师）资助基金项目：国家973计划项目2004CB518805军队“十一

3、五”科技攻关项目(06G084)教育部“长江学者和创新团队发展计划”创新团队IRT0459关键词：HV；单链抗体；鱼精蛋白；靶向输送中国人民解放军第四军医大学2008年05月第四军医大学硕士学位论文目录缩略语表·····················································································································1中文摘要·················································

4、·········································································3Abstract·····························································································································5前言··································································

5、························································7文献回顾·····················································································································9（一）、汉坦病毒（HV）感染疾病治疗研究进展·····················································9（二）、单链抗体融合蛋白在生物治疗中的应用

6、····················································12（三）、靶向性非病毒载体系统介导的基因转移····················································17（四）、siRNA的研究进展························································································21正文·········································

7、···············································································24第一部分抗HV单链抗体/Cκ/protamine截短体融合蛋白的基因构建·································241.实验材料······································································································241.1菌种与质粒······

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