黑曲霉植酸酶微丸的制备和质量评价

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1、：0：一．。I黑龙江大学硕士学位论文IIAbstractPhytaseservesasabiologicalfeedadditiveforelevatingtheutilizationlevelofphosphorusinfeed,improvingperformanceofanimalproduction,andreducingenvironmentalwaterpollutioncausedbythehigh—phosphorusdejectionofanimal．Buttheapplicationandspreadofphytasehavebeenlimited

2、inChinabecauseofitspoorantireversionforce，especiallyitspoorthermalstability．Inthisthesis，wepreparedakindofpelletofphytaseofAspergillusnigerwithgoodthermalstabilityusingadvancedtechnologyofpharmaceuticalpreparations，andevaluateditsquality．ThisstudywasdesignedtoprepareakindofphytasepeHets

3、ofAspergillusnigerwhichhaveagoodthermalstabilitybyapplyingofadvancedtechnologyofpharmaceuticalpreparations．Theactivityofphytasepelletswerequantifiedbyvanadium—ammoniummolybdatemethod．Theresultsofintra-dayandinter-dayprecision,recoveryandsampledeterminationweresatisfying，provingthatthism

4、ethodisascientific，reliableandsimplemethodforquantitativeanalysisofphytase，andCanbeusedforactivitydeterminationofthephytasepelletsduringinspectionofpreparationandstability．Wepreparedphytaseprimepelletspossessingsmoothsurfar：e，900droundness，small-friability,uniformparticlesizebyusingextr

5、usion-spheronizationmethodwithhighyield．ThegoodliquidityoftheobtainedpelletsWasinfavorofitsmixingintofeedpelletsandapplyingintoactualproduction．LiquidityofpelletsWasgood，SOitisconducivetomixingintofeedpelletsandapplyingintoactualproduction．Phytasecoatedpelletswerepreparedbycoatingphytas

6、eprimepelletsusingbottom—spraytypefluidizedbed．Therelativeresidualactivityofphytasecoatedpelletstreated诚msteamheat-moistureat100℃for2minservedasthemaininspectionindicators．Wedeterminedthecoatingprescriptionbyselectingthemannerofcoating，coatingmaterialofeverylayerandthetotalweightaSwella

7、sweightratioofcoatinglayeraccordingtoactualproductionrequirementsandstudiedtheeffectofprocessfactorsonthermalstabilityofphytasecoatedpellets．Theresultsshowedthatqualityandcharacterstudyofphytasecoatedpelletsmettherequirements，thephytasecoatedn●Abstractpelletshaduniformactivity,