雌激素h.pylori对人十二指肠粘膜碳酸氢盐转运蛋白的影响

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1、遵义医学院硕十学何论文雌激素与H．pylori对人十二指肠粘膜碳酸氧盐转运蛋白的影响岁女性及60．70岁男性(尸O．05)。Western-blot结果显示：20．30岁女性CFTR蛋白的表达量高于20．30岁男性、60．70岁女性及60．70岁男性(PO．05)。20．40岁慢性胃炎(Hp+)患者和十二指肠溃疡患者(Hp+)比对照组(Hp．)十二指肠球部粘膜中SLC26A6的mRNA表达降低(尸

2、mRNA表达无明显差异(尸>0．05)。结论：雌激素调节了人十二指肠粘膜碳酸氢盐转运蛋白CFTR得表达，而对SLC26A3、SLC26A6没有影响，此结果有助于阐明雌激素调节碳酸氢盐分泌的机制有一定帮助。H．pylori影响了十二指肠粘膜碳酸氢盐转运蛋I刍SLC26A6的表达，这一结果为H．pylori侵袭粘膜导致溃疡发生的机制提供了理论依据。关键词：雌激素：H．pylori：人：十二指肠粘膜；碳酸氢盐转运蛋白；囊性纤维化跨膜传导调节因子：离子交换体26A3：离子交换体26A6遵义医学院硕十学何论文雌激素与H．pylori对人十二指肠卡占膜碳酸氧盐转运蛋白的影响Influenceofes

3、trogenandH．pylorionhumanduodenalmucosalbicarbonatetransportproteinABSTRACTBackgrounds&Purpose：Duodenalmucosalbicarbonatesecretionisallimportantprotectivefactorofduodenalmucosaagainstacid-inducedinjury．Inthisstudy,weinvestigatedtheinfluenceofestrogenandH．pylorionhumanduodenalmucosalbicarbonatetran

4、sportprotein．Weexpectedto触erelucidatethecellularmechanismswherebyestrogenincreasesduodenalmucosalbicarbonatesecretionandpathogenesisofH．pylori-relatedduodenalulcer．Methods：Healthyfemalevolunteersinthe20-30yearswithoutH．pyloriinfectionconfirmedbyC·14breathtestwereselected．Duringtheirovulatoryperio

5、dsandmenstrualperiodswhichisconsideredasthepeakandlowvaluesofestrogenproductioninfemalemenstrualcycle,duodenalmucosaWastakenbyendseopicbiopsyformRNAexpression锄alysisofduodenalmucosalbicarbonatetransportproteinCFTR，SLC26A3，andSLC26A6andverlousbloodWastakenforserumestrogenmeasurement．Inaddition,hea

6、lthyfemalevolunteersinthe20-30yearsand60-70years，andhealthymalevolunteersinthe20-30yearsand60-70yearswerealsoselected．AllsubjectswereH．pylori—negativewhichwasconfirmedbyrapidill'easetest．DuodenalmucosawastakenbyendoscopicbiopsyformRNAandproteinexpressionanalysisofCFTR，SLC26A3，andSLC26A6．Selecting

7、patientswithH．pylori-infectedduodenalulcerandH．pylori-infectedchronicgastritisintheageof20-40years，andhealthycontrolswithoutH．pylofiinfectionintheageof20-40years，duodenalmucosaWastakenformRNAexpression锄al”isofCFTR，SLC2