中国荷斯坦牛c4a基因的遗传变异与其作为乳腺炎抗性标记的可行性分析

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1、ABSTRACTSTUDIESoNGENETICV

2、ARIATIoNoFC匀嗜GENEANDITSFEASIBILITYASMOLECULAR№～RKEROFMASTITISRESISTANCEINCHINESEHOLSTEINCATTLEComplememcomponent4(C4)isacriticalcomponentinthecomplememactivityandprotectionagainstmanybacterialpathogensbecauseitisessentialtotheclassicalandlectinactiva

3、tionpathways．TheserumconcertrationofC4islowerthanthatofC3，andrankssecond．C4isexpressedprimarilyintheliver，butC4mRNAhasalsobeendetectedintheheart，lung，spleen，kidney，brain，thyroidandmammarygland．C4hashi曲polymo印hismsandlinkstothemajorhistocompatibilitycomplex，whichshowssignifica

4、ntassociationwitllmastiffssusceptibility．Therefore．itisveryimportanttostudythegeneticvariationofbovineC4Ageneanditsassociationwithmastiffsresistance．1．PolymorphismofC4AgeneanditscorrelationwithmilkproductiontraitInthecurrentstudy，thebovinecomplememC4AgeneWasscreenedforpolymor

5、phisms，andtheassociationsofthesepolymorphismswiththehemolyticactivityoftheclassicalpathway(CH50)，serumC4levels，andmilkperformancetraitswereexamined．Threenovelsingle-nucleotidepolymorphisms(g．2994A>G，g．3508A>Gandg．3649G>C)weredetectedbyDNAsequencingandPCR-RFLPin182ChineseHolst

6、einCOWS．Theg．2994A>Gmutationinexon10ledtomethionineandvalineexchangeatposition362，whereasg．3508A>Gandg．3649G>Cweresynonymoussubstitutions．ThestatisticalanalysesrevealedthatCOWSwithg．2994A>G-AGandg．3649G>C-CChadsignificantlylowersomaticcellscores(SCS，P<0．01)．HomozygoteCOWSwith

7、GAChaplotypeshadthelowestSCS，whereasAAG／AACCOWShadthehighest．TheserumconcentrationofC4byELISAandthehemolyticactivityandantibacterialactivityofCH50werealsoevaluatedintheIII南京农业大学硕士学位论文中国荷斯坦牛C4A基因的遗传变异及其作为乳腺炎抗性标记的可行性分析currentstudy．Theserumfromgeneticallyresistant(H5H5)animalsdi

8、splayedasignificantlyhigherantibacterialactivityagainstSta．aureusandE．coliinvitrocompared谢tlltheserumfromgeneticallysusceptibleH2H1(P<0．001)．Moreover,nosignificantdifferenceinC4levelwasobservedinthreeSNPsintestedpopulations．TheindividualswithH5H7showedahigherC4levelthantheone

9、swithH5H5伊