造血干细胞移植过程中adamts13与vwf的检测与其临床意义

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1、AbstractClinicalsignificanceofADAMTSl3andVWFinpatientsf01lowinghematopoieticstemcelltransplantationClinicalsignificanceofADAMTSl3andVWFinpatientsf0UowinghematopoieticstemceUtransplantationObjective：ToestablishastablemethodofADAMTSl3actiVitydetecting．ToobservethechangesofA

2、DAMTSl3actiVityandVonWillebrandfactor(VWF)antigeninpatientsfollowinghematopoieticstemceUtransplantation(HSCT)inVariousperiods．ThentoanalyzethechangesandiIne：ractionbetweenADAMTSl3andVWFinVariousperiods，andtoinvestigatetheclillicalsigllificanceofADAMTS13andVWFinpatientsfol

3、lowingHSCT．Methods：(1)Fluorescenceresonanceene昭ytransfersubstrateVWF73(FRETS_VWF73)assaywasestablishedtodetecttheplasmaADAMTS13actiVityin18patientswithidiopamicthrombotict11rombocytopeIlicpu叩ura(TTP)．Theresidualcollagenbindingassaywasusedatthesametime．TWentymVehealthydono

4、rswereeIlrolledintocontrol伊oup-(2)Patients：Atotalof113patients(66males，47females)receiVingHSCTwereinvestigated舶mSep2010toSep2011inourhospital．Medianageis32(4_57)years01d，including39caseswithacutemyeloidleukemia(AML)，24caseswimacutelymphoidleukemia(ALL)，16caseswithc11ronic

5、myelogenousleukeIIlia(CML)，4caseswithacuteheterozygouscellleukemia(AHL)，13caseswithlymphoma，17caseswithomerdiseases．Accordingtothedonortype，16casesreceiVedautologoustransplantation，97casesreceivedallogeneictransplamation(49casesfromsiblingmatcheddonors，30cases行omuIlrelate

6、ddonor，1Ocases仔ompartiaUymatchedfaIIlilydonors，9casesfromumbilicalcordbloodstemcelltransplantation)．ThemodifledBU／CYorTBI／CYwasusedf-0rconditioningregimeninmostpatients，theBEAMornonmyeloablatiVeregimenwasusedinmerestCaSeS．(2)Samplecollection：Thesodiumcitrateanticoagulated

7、plasmawascollectedinpatientsIVclinicalsigIlificanceofADAMTsl3andVwFinpatientsfouowinghe肌ltopoieticstcmcen仃粕spl柏tationAbstractfollowingHSCTinVariousperiods．TheADAMTS13actiVityandVWFantigenweredetectedwithFRETS二VWF73andELISA．(3)Statisticalanalysis：UsingSAS9．3datawasexpresse

8、dasmean±standarddeviation．Datadiff矗entwasanalyzedusinganalysisofVarianceandftest．Rateswerecompar