通心络对局灶性脑缺血大鼠微血管新生与其代偿作用的实验分析

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1、英文摘要ExperimentalstudyontheinterVentionalefl’ectsof1’0ngxinluoonangiogenesisandcompensatoryfunctionafterfocalcerebral●■lSCnemlaABSTRACTobjective：Inthisstudy，directedbycollateraldiseasetheo拶，usingcraniotomy1igationasideofthemiddlecerebralane巧establishedthemodeloffocalce

2、rebralischemia．1bexploreen’ectiVelytherapeutic如nctiononneurologicalrecoVe巧ofischemicstroke，wechoseitsrepresentatiVeprescription：Tongxinluo，andobserveditsimerventionaleffectsonbehaVioralscoring，mo叩h0109yofbraintissue，microVasculardensi吼diameterandvolumesofbloodpermsion

3、，relatedangiogenesisfactorsandtheirexpression．Theresultswouldbehelp如lfor如rtherproVidingexperimentalsupportforscientincvalueofcollateraldiseasetheoWinischemiccerebroVasculardisease．Methods：1Thee位ctsofTongxinluooninf．arctionareaandneurologicalbehaVioroff．ocalcerebralisc

4、hemiaratsFocalcerebralischemiamodelwasdevelopedwithmaleSDratsofhealthSPFgrade．TheanimalswererandomlydiVidedintosixgroups：theshamgroup，themodelgroup，Tong)【inluohi曲一dose(2．49／l(g。d)黟oup，middle．dose(1．29／kg·d)group，10w-dose(O．69／kg·d)groupandthenimodipine(1Omg／1喝·d)group

5、．Usingcraniotomyligationasideofthemiddlecerebralarteryestablishedthemodeloffocalcerebralischemia．Shamgroupwasonlypushedasidemeninges，notligationVascular．Allgroupswereirrigated，besidessh锄groupandmodel铲．oupweregaveisotonicNachloride．TheneachgroupwasdiVidedintothreetimep

6、oints：3d，7dand14d(n。6)．PosturalI沁nexTest，ForelimbMuscleStrengthTestandBeamWalkingTestwereusedforneur0109icalbehaviorgrading．HEstainingwasusedtodetectthehistomo印hologicalchangesofbraintissueandTTCStainingwasusedto5英文摘要calculatetheproportionofcerebralinf打ction2urea．2The

7、effectsofTongxinluoonmicrovasculardensi吼di啪eterandVolumesofbloodper如sionGroupingandmodelingidem．Microvasculardensityofcerebralcortexischemiaareawastestedthrou曲immunohistochemicalkitat14daRerheartpermsionfixation．MicroVasculardiameter，mo巾hologyandvolumesofplasmapermsio

8、nofcerebralmicroVessels，throughfemoralVeinbeingper如sedwithFITC—deXtran，wereanalyzedwimlightmicroscope(40times)at14daRerfocal