小肠黏膜下层组织和丝素蛋白2f壳聚糖大孔微载体复合骨髓间充质干细胞修复兔耳廓软骨缺损

《小肠黏膜下层组织和丝素蛋白2f壳聚糖大孔微载体复合骨髓间充质干细胞修复兔耳廓软骨缺损》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、硕士学位论文umbilicalbloodmesenchymalstemceilsfromfull·termnewborn’Sumbilicalvein．Throughthedensitygradientobtainedfromumbilicalcordbloodumbilicalcordbloodmesenchymalstemcellsandculturedinvitro，ThestatisticsweredealedwitllstatisticalsoftwareSPSS13．0．Theinter-groupdiffere

2、ncesoftheentirecellcycleanalysisofSinglefactorrepeatedmeasures，thelawofcellsexpressisshowedbyinearmap．HumanumbilicalbloodmesenchymalstemcellsmarkedbyBrdU，rabbit’Schondrocytesinducinghumanumbilicalbloodmesenchymalstemcells、析ththetechnologyofTranswellnon-contacteo-cu

3、lture，andthenanalyzedbyimmunocytochemistrymethod．四．Themethodofallograftbonemarrowmesenchymalstemcells／smallintestinalsubmucosacllogeneicomplextorepairdefectsofrabbitearToinvestigatethepossibilityofusingchondrocytesthatcreatedfrombonemarrowmesenchymalstemcells(BMSCs

4、)whichinducedbytransforminggrowthfactor-131asgermcellsandSISasbrackettoformnewcartilageandrepairtherabbits’earcartilagedefects，at4，10，and16weeksaftercultivation,andthesampleswerestainedwitllhaematine·eosin，toluidineblueandMasson’Strichrome．generalobservationandhist

5、ologicalexaminationwereconductedonthesamplesofbonedefectarea，Toknowthesituationofallogeneicbonemarrowmesenchymalstemcells／smallintestinalsubmucosathedefectofauriclerepairedforrabbit五ThemethodofallograftbonemarrowmesenchymalstemcellsandSF／CScomplextorepairdefectsofr

6、abbitearAttemptedtoselectsilkfibroinandchitosanmixedinaccordancewithacertainproportion，byemulsification-Chemicalcross—linking，freeze—drying，polarsolutionPreparationhandlewitllaporousstructureofSF／CSmacroporousmicrocarriersforbonemarrow-derivedmesenchymalcells．SF／CS

7、macroporousmicrocarriercomplextocarryoutinvitroandanimalinvivoobservation；Toobservethesituationofcellgrowthing、析thgraftbyinvertedABSTRACTmicroscope，andthenimplantethecomplexontherabbiteardefects7dayslater,8，12weekslatergothistologiesinlinegeneralobservationandHEsta

8、ining，Massoffstrichromehistologicalobservation．Result：一．Bonemarrowmesenchymalstemcellswereseparatedandpurifiedbyusingattachmentculturemethod．Thet