资源描述：
《人脐带间充质干细胞对肺纤维化模型的治疗作用研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、重庆医科大学硕士研究生学位论文(HYP)ofPFmicelungwerealsoincreasedgradually,especiallyafter21dBLMinjection，thesedifferencesbetweencontrolmiceandPFmicewerethesignificantlybiggest.Conclusion:BLM-inducedPFmodelweresuccessfullyestablished,andthemodelshouldbemuchmorestableafter21dBLMin
2、jection.Keywords:Bleomycin;Pulmonaryfibrosis;HYPPARTⅡTHEEFFECTOFHUC-MSCSONPULMONARYFIBROSISMICEObjective:TodeterminethebesttimethattheeffectofHUC-MSCsonpulmonaryfibrosismice,andtoexploretheroleandthepotentialmolecularmechanismofHUC-MSCsonPFmice.Mothods:C57BL/6mice
3、weredividedintofourgroups(PBS+PBS,PBS+HUC-MSCs,BLM+PBS,BLM+HUC-MSCs).ThemousemodelofPFwasinducedbyintratrachealinstillationBLM.Intraperitonealinjectionofanesthesiabyforendotrachealinstillation.1hor12hor24hafterBLMinstillation,micewereintratracheallyadministeredwit
4、hHUC-MSCsorPBS,andtheirlungissueswerecollectedatpost21days.H&EandMassonstainingoflungtissuepathologyandpathologyscoresweretodeterminethebesttimethattheeffectofHUC-MSCsonpulmonary6万方数据重庆医科大学硕士研究生学位论文fibrosis.AtthebestpointintimetogivecellsorPBS,lungissuesandBALFwer
5、ecollectedat21days,theconcentrationsofHYP,TGF-β1,TNF-α,IFN-γandIL-1βinBALFweredetectedbyELISA.HUC-MSCswerelabeledwithCM-Dil.TheHUC-MSCstransferenceandresidencetimeinpulmonaryweredetectedat1h,24h,7d,14dand21dthroughinstillingthelabelingcellsintratracheally.Results:
6、1hor12hor24hafterBLMinstillation,micewereintratracheallyadministeredwithHUC-MSCsorPBS.TheresultsshowedalveolarinflammationandcollagenstainingwassignificantlyreducedintheHUC-MSCstreatmentgroup,andtheBLMinstillation1haftergivingHUC-MSCswasoptimaltherapeuticeffectsan
7、dthebesttherapeutictime.ThedatashowedthatmicewithHUC-MSCstreatedweredemonstratedtherelievedclinicalmanifestationsofmortality,weightloss,andlackofoxygen.TheinflammationandbleedingofalveolarwasreducedbyHEstaning,collagendepositionofMassonstaining.TheconcentrationofH
8、YP,TGF-β1,TNF-αandIL-1βinBALFofthemicetreatedwithHUC-MSCsweredecreased,whileIFN-γwaselevatedbyELISA.HUC-MSCscouldbelabledbyCM-Dilwithhighefficiency.Thed
