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1、汕头大学医学院硕士学位论文cellsusingthepreviouslydescribedprotocolforJNKactivityassay.4.DifferentconcentrationofJNKinhibitorshadnoeffectontheexpressionofp53eitherinthewtcellsorcrt-/-cells.Interestingly,p53inhibitor(50uom/l,80umol/l)significantlydecreasedtheexpressionofJNKinthewt
2、cellsinconcentration-dependentmanner.Meanwhile,theexpressionofJNKinwtcells,whichevenexposingtothehighdoseofPFT-α(80umol/l),wasstillhigherthanthatincrt-/-cells.However,differentconcentrationofPFT-αhadnoeffectontheexpressionofJNKincrt-/-cells.Conclusion1.ObviousERSwas
3、showninH9C2cellstreatingwithH2O2for4hour-period.However,otherERSrelatedproteinGRP78wasnotchangedsignificantly,whichmayduetotheactionoftimeandstrengthisnotenough.Atthesametime,theactivityofMMP-2andMMP-9werechanged.Toassumetheconclusionweneedmodulatethetimeandconcentr
4、ationofH2O22.ThereisnosignificantJNKactivitydifferencebetweenwtandcrt-/-cells.3.Theexpressionofp53proteinbothincrt-/-cellsandwtcellsmaynotrelatedwithJNKpathway.Itsuggestedthattherewasapositivefeedbackloopbetweenp53andJNK.KeywordsEndoplasmicreticulumstress,Calreticul
5、in,Matrixmetalloproteinase,JNKV汕头大学医学院硕士学位论文英文缩略词英文缩写英文全称中文名称EREndoplasmicreticulum内质网ERSEndoplasmicreticulumstress内质网应激UPRUnfolded-ProteinResponse未折叠蛋白反应CRTCalreticulin钙网蛋白GRP78Glucose-regulatedprotein78糖调节蛋白78IRE1insulin-responseelement1胰岛素反应元件1ATF6activatingtrans
6、criptionfactor6胰岛素反应元件1PERKproteinkinaseR-likeERkinase蛋白激酶R样内质网激酶ERSEEndoplasmicreticulumstress内质网应激反应原件responseelementMMPsMatrixMetalloproteinases基质金属蛋白酶ECMextracellularmatrix细胞外基质MMP-2MatrixMetalloproteinase-2基质金属蛋白酶-2MMP-9MatrixMetalloproteinase-9基质金属蛋白酶-9MT-MMPs
7、Membrane-typeMMPs膜型基质金属蛋白酶MAPKmitogen-activatedproteinkinase丝裂原启动蛋白激酶JNKc-JunNH2-terminalkinasec-Jun氨基末端激酶HFheartfailure心力衰竭MDM2murinedoublemimute2泛素蛋白连接酶FBSfetalbovineserum胎牛血清Rpmrevolutionperminute转/分钟hhour小时minminute分钟℃centi-degree摄氏度DMSODimethylsulphoxide二甲基亚砜TE
8、MEDN,N,N,N,-tetramethyl-ethylene四甲基乙烯二胺VI汕头大学医学院硕士学位论文PAGEpolyacrylamidegelelectrophoresis聚丙烯酰胺凝胶电泳TBStris-bufferedsalineTris缓冲盐溶液SDSsodiu
