鲢鱼蛋白酶解物的功能性质、抗氧化活性与其ace抑制肽的制备

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1、qualityproteinbecauseoftheresultshoweditrichinessentialaminoacidlikeLeucine,LysineandValine．Inconclusion，SCPcouldbeusedforpreparingproteinenzymehydrolysis·AlcalasewasthemostsuitableforhydrolysisinthesixkindsoIproteases(Trypsin，Flavourzyme，Neutrase，Papain，Protamex，Alcal

2、ase)，itwasscreenedouttohydrolyzeSCEThehydrographofSCPHhydrolyzeshowed：TheDHincreasedwithtime，thehydrolysisratedecreasedgradually．DHcouldbecontrolledbycontrollingreactiontime，SOtheSCPHindifferentDH(4．5％，9．O％．13．5％，18．O％)couldbeobtained．(2)ComparedwithscP,thefunctionalpr

3、opertiesofSCPHindi艉rentDHwe化betterexceptoilholdingcapacity．Meanwhile，pHcouldaffectthesolubilityandemulsifyingproperty．Specificresultswereasfollows：ThesolubilityofSCPandSCPHcangrowupwiththeincreaseofDH，thenitrogensolubilityindex(NSI)ofSCPHindifferentDHimprovedduringpH2．

4、0—10．0，theNSIofSCPHinpH7．0enhanced77·7％ofSCP．WaterholdingcapacityincreasedasDHincreasing’thewaterholdingcapacityofSCPHinDH18．O％improved37．7％toSCEWhereas．oilholdingcapacitydecreaseasDHincreasing．Emulsifyingactivityindex(EAI)andEmulsionstabilityindex(ESI)ofSCPwerebetween

5、SCPHinhighDHor10wDH，themostoutstandingemulsifyingpropertyofSCPHturnsupinDH4．5％．theEAIofSCPHinDH4．姒wasdeterminedas58．8％higherthanSCPinpHIO．0．ThefoamingabilityofSCPandSCPHcouldreachthebestvalueinDH4．5％．whichwas20．4％higherthanSCEGoodfunctionalpropertieswerethepremisethatt

6、heactivepeptidecouldbepreparedforfoodsandfoodadditives．(3)TheantioxidativeactivityofSCPHwasobviouslybetterthanSCPinfourkindsofantioxidantsystems．SCPHwithDH13．5％exhibitsbetteractivitiesthanthosewithotherthreeDHvalueinDPPHradicalscavenging(DPPH．)，superoxideradlcalscaveng

7、ingandreducingpowerradicalscavenging．TheDPPH．inhibitionratioreachedthemaximalDPPH·inhibitionratioofvalueof39．95％at1．60mg／ml，it's7．1timeSofTheSCPH，ThereducingpowerofSCPHinDH13．5％V(4)OnthebasisofsinglefactorexperimentwhichtakingDHasindex，theoptimizehydrolyzationcondition

8、swasperformedbytheresponsesurfaceanalysis：pH8．73，T54．7℃，[E]／[S]I．78％．TheACEinhibitionratiooftheSCPHpreparedwiththeopt