胆汁酸在大口黑鲈饲料中有效性与耐受性评价

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1、万方数据山东农业大学全日制硕士专业学位论文EfficacyandToleranceAssessmentofBlieacidinMicropterussalmoidesAbstractTwoexperimentswereconducedinourresearch．Theeffectsofbileacidongrowthperformance，haematologicalprofilesandantioxidantintestinalenzymeactivity,bileacidcontentintheliver,intestinesandgallbladder，theintest

2、inalhistologyoflarge—mouthbass(Micropterussalmoides)WaSinvestigated，todeterminetheefficacyandtoleranceassessmentofbileacidinaquaticfeeds．1．Efficacyassessmentofbileacidinlarge-mouthbassdietsSixexperimentaldietswaspreparedwithbileacidlevelsat0、80、160、240、300、600mg／kg，respectively．Eachdietwasfe

3、dtosixreplicatesof30large-mouthbass(6．174-0．02曲for70days．heresultsshowedthattheadditionofbileacidhadsignificanteffectsongrowthperformanceoflarge-mouthbass．300mg／kgbileacidimprovedSGR,WGRandFRsignificantly．240，300mg／kgbileacidincreasedbodyfatoflarge—mouthbasssignificantly．300meCkgbileacidincr

4、easedSOD，T-AOCinplasma,andT-AOCintheliver,decreasedMDAintheliver．Withtheincreasingadditionofbileacid，GSH-PXinplasmaandliverincreasedsignificantly．Inaddition，totalproteinenhancedsignificantlywithadditionofbileacidatlevelsof300and600mg／l(g．Theadditionofbileacidalsoimprovedamylaseactivityinante

5、riorandmidintestines．Moreover,withtheincreasingadditionofbileacid，bileacidingallbladderincreasedsignificantly．OnthebasisofSGlLFRand‘FBAintheliver,theoptimaldietarybileacidlevelswere440．5mg／l(g，283．3mg&gand253．8mg／kgrespectivelyusingbroken—linemodel．Varianceanalysisshowedthatlarge—mouthbaSsob

6、tainedbestgrowthperformancewithbileacidlevelat300mg／kg．Withinthescopementionedabove，bileaciddecreasedMDAintheliverandimprovedantioxidantactivity．BileacidsignificantlypromotedfatmetabolismviaincreasingI-IDL—Ccontentinplasma．Moreover,theadditionofbileacidenhancedamylaseactivityinintestines．Toc

7、onclude，therecommendeddoseofbileacidinlarge—mouthbassdietwas253．8-283．3mg／kgandthehighestrecommendeddosewas300mgCg．2．Toleranceassessmentofbileacidaddedinlarge·mouthbassdietsFourexperimentaldietswerepreparedwithbileacidlevelsat0，300，600，1500mg／kg，in