靶向sirna体外抑制肝癌hepg-2细胞vegf-b基因表达的分析

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1、英文摘要_————————————————————————————————————————————一TheinhibitionofVEGF．BbytargetedsiRNAinVitroliVercancerceIIsHepG一2ABSTRACTobiective：Primary1ivercancerisoneofthemostcommonmallgnamtumorsinourcount巧，surgicalresectionisthepreferredchoiceand也emostef．fectivemethods．But，meclinicals舯ptoms

2、ofliVercancerappe2urlate，manypatientswithlivercanceraremiddleorad、r狐cedstagewhensymptomsshowup，rateofsu唱e巧islow，prognosisispoor．MetastaslsandrecurrencelsthernostirnpommtreasontoreducesuⅣiValtimeofpatientswithliVercancer·GrowthandmetastaSisoftumordependonanglogenesls·Vauscularendome

3、lialgrowcllfactorfamilyme瑚【bers(VEGFs)canenCourageangiogenesisdirectlyorindirectly．Sof打，thereareseVenmembersmVE(j

4、s，include：VE(讯．A，VEGF．B，VEGF．C，VEGF-D，ViralVEGF(VEGF-E)，snakevenomVEGF．(ⅦGF—F)andPLGF(placenta伊oWthfactor)·ExceptVEGF．EandVEGF．F，omerⅦGFsmembersareaUencodedbym锄mals贴ne．

5、VEGFsarewidelyexpressedinVertebrateanimals，andt11ecodiI培sequenceishi曲lyconservativeindifferentSpecies．VEGFmembersandits’receptors2ureoverexpressedinmanytumortissue，especlallymtheturllortissuew油morebloodvessels(suchasliVercancer)，there1sacIoserelationshipbetweenVEGFsme瑚【bersandchara

6、cteristicsoftumorsuchaSmetastasisandrecurrence．AsoneofVEG，Fmenlbers，VEGF-Bex弘essmghlyinvarioustumortissueandplayanimportantrolemtumor’smVaslonandtransfer．OurpreVious咖dysuggestedthatⅦGF-Bplayedanimportantr01einlivercancerlsinvasion锄dmetastasis，锄dhaVerelatedcloselywiththeprognosisofp

7、atientswithlivercancer．Therefore，ItisexpectedtoinhlbltllVercancercell’sproliferationandmetastaSisbyrestrainingVEGF-Bexpress·RNAinterference(RNAinterference，RNAi)isanewtechnoIogydeVelopedmrecentyeaurs，silencedspecmcgeneinViVoorinVitro．SmallinterferingRNA(ofsmallinterferingRNA，siRNA)

8、ismemainef-fectorsofRNA英文摘要imerference．Certaingenecanbesilencedef-fectiVelybytargetedsiRNA．IIl“s咖dy，wereconstmctplasmidcarrieroftargetedVEGF-BsiRNAinVitro，thentransfecttheVectorcontainingmeobjectgeneintoliVercancercelllineHepG一2cell，usereVerset啪scription—polymerasechainreaction(I汀-

9、PCR)todetectVEGF-Bm剐NAexpr