马尔尼菲青霉菌双相转换相关基因amyr和ctf1β功能的初步分析

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1、马尔尼菲青霉菌双相转换相关基因AmyR和ctf1β功能的初步研究2016届学术型硕士研究生论文前言...............................................................................................................241材料和方法................................................................................................271.1实验菌株.............

2、..............................................................................271.2实验试剂和仪器..............................................................................271.3实验方法...........................................................................................342结果与分析..........

3、......................................................................................552.1马尔尼菲青霉菌AmyR、ctf1β基因缺失突变载体的构建...........552.2马尔尼菲青霉菌AmyR和ctf1β基因缺失突变体的初步筛选.....572.3荧光定量PCR进行转化子筛选鉴定和外源基因插入拷贝数检测....................................................................................

4、.............................582.4荧光定量PCR对AmyR、ctf1β转录因子基因相关基因表达量的检测.........................................................................................................602.5基因突变菌株RAW264.7鼠巨噬细胞实验..................................622.5△AmyR基因突变菌株的淀粉水解实验结果............................

5、.....672.6基因突变株药物敏感、抗氧化、细胞壁完整性和渗透压敏感性实验结果.................................................................................................693讨论...........................................................................................................71参考文献..............................

6、...............................................................................73综述.....................................................................................................................77摘要..................................................................................

7、.............................77结语...............................................................................................................87万方数据马尔尼菲青霉菌双相转换相关基因AmyR和ctf1β功能的初步研究2016届学术型硕士研究生论文参考文献...................................................................................