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ID:32045295
大小:1.68 MB
页数:45页
时间:2019-01-30
《大肠埃希菌耐药性与耐药基因检测》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、33株,检出率为70.4%(38154)。7.产ESBLs组携带qacEAl.sull基因菌株数为20株,不产ESBLs组携带qacEAl-sull基因菌株数为l8株。(X2=0
2、355,P>0.05)。结论:I.ESBLs阳性大肠埃希菌水解头孢噻肟优于头孢他啶。2.除氨基耱苷类抗生素和呋喃妥因外,产ESBLs组耐药性明显高于非产ESBLs组。3.产ESBLs大肠埃希茵中TEM型为最常见的D.内酰胺酶型别。4.氨基糖苷类耐药基因以aac(3)-II和aae(6)一I为主。5.喹诺酮耐药率较高,且检测到qnr基因。6.qacEAl-sull基因携带率较高
3、。7.产ESBLs组与非产ESBLs组大肠埃希菌qacEAl.sull基因携带情况的差别不具有统计学意义。‘.关键词:大肠埃希菌超广谱13-内酰胺酶耐药性基因型耐消毒剂基因Thedetectionofdrug-resistanceandresistancegeneofE.eoliPostgraduate:PanJingTutor:KouXinmingMajor:LaboratoryMedicineDiagnoseAbstractObjective:1.toinvestigatethedrug—resistanceproducedbyEschericbi
4、acoli(E.coil)fromourhospitalandthedifferenceofdrug—resistancebetweenextended-spectrump—laetamases(ESBLs)positiveandextended-spectrumB—lactamases(ESBLs)negativegroups.2.toinvestigatethegenotypesdistributionofESBLsproducedbyE.eoli.3.toinvestigatethedrug—resistancerateanddrug·resis
5、tancegeneinE.coliofaminoglycosidesandquinolones.4.toinvestigatethedisinfectant—resistancegene(qacEA1·sull)inE.coliandthedifferencebetweenthetWOgroups.Method:1.27strainsofESBLspositiveand27strainsofESBLsnegativeE.colifromYanTaiYuHuangDingHospitalfromSeptember2005toJune2006werecol
6、lected(54strainsnonrepetitiveE.coilintotal).2.K—Btestwasusedtodetectdrug-resistanceofE.colito22antibiotics,theresistantrate(R%)、intermediaterate(I%)andsensitiverate(S%)werecalculatedaccordingtothecriteriainguidelinesofCLSI/NCCLS(2005).3.PCRWaSusedtoamplifyESBLsgene、aminoglycosid
7、es-resistancegene、quinolones-resistancegeneandqacE△1-sullgenetoindentifytheirgenotypes.Results:1.thesusceptibilityofESBLspositiveE.colitoCeflazidimeandCefotaximewere66.7%(18/27)and3.7%(1/27)respectively.2.therelativitybetweenthetwogroupstoaminoglyeosidesandnitrofurantoinresistan
8、ce,P<0.05,andtotheothers,P>0.05.3,among27strainsofESBLspositiveE.coli,CTX,M—1wereamplifiedfrom9isolates,,SHVwereamplifiedfrom5isolates,TEMwereamplifiedfrom16isolates,andOXAWereamplifiedfrom8isolates..MorethantwotypesofESBLsgenewereproducedbytenofthem.PER、VEB、GES、CARBwerenotfound
9、among27strains,4,44strainsWereresistanttoaminog
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