次氯酸钠溶液冲洗根管对粪肠球菌的作用分析

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1、EfficacyofsodiumhypochloriteasendodonticirrigationinreducingEnterococcusfaecaliswithinrootcanalsMasterdegreecandidate：JingzhiZhangSupervisor：ProfessorWeidongNiuMajor：StomatologyAbstractObject：Thisexperimentevaluatestheefficacyof5．25％sodiumhypochlorite(NaClO)ase

2、ndodontieirrigationsolutionagainstEnterococcusfaecaliswithinrootcanals．Method：Atotalof20extractedhumanmandibularpremolarteethwithasinglerootcanal，weredealedwithstandardtechnique．Theteethwereplacedintoglasstubescontainingenterococeimediumandautoclaved·Therootcan

3、alsoftwentyextractedteethwereinfectedbyEnterococcusfaecalisf疗’，it，．D．andthenculturedinenterococcimedium．TheteethwereinstrumentedusingsterilestainlesssteelK—filesuptosize40withstandardizationTwentyextractedhumanpremolarswererandomlyandevenlvdividedinto5．25％sodiu

4、mhypochlorite．O．9％sterilesalinesolution(NaCl)，eachgroup10premolars．Canalsweresampledafterinstrumentationusingsterilepaperpoints，andthenincubatedat37"C·Thecolonyformingunits(CFU)grownwerecountedat24h，48handlweek··ResuIt：At24hour，48hour，Enterococcusfaecalisin5．25

5、％sodiumhypochloritegroupwerecompletelykilled，andthecolonyformingunitswere0CFUml．1．TheaveragecolonyformingunitsoftheNaCIgroupwererespectively1．2x106CFUml～，1．7x106CFUml～．Thereweresignificantdifferencesbetweenthetwotestedgroups(P<0．01)．At1week，fewEnlefococcusfaeca

6、lisweredetected．TheaveragecolonyformingunitsOtthe5．25％sodiumhypochloritegroupwas109CFUml。‘andtheNaCIgroupwas3．8x106CFUml～．Thereweresignificantdifferencesbetweenthetwotestedgroups(P

7、elykillEnterococcusfaecalis，butitnotcleanuptheEnterococcusfaecaliscompletely．SodiumhypoehloriteonlyreducetheamountoftheEnterococcusfaecalis，andmakeitinbacteriostasiswithinaperiodoftime．Keywords：sodiumhypochloriteEnterococcusfaecalisendodontieirrigationplatecoun

8、t次氯酸钠溶液冲洗根管对粪肠球菌的作用研究硕士研究生：张敬之指导教师：牛卫东教授专业名称：口腔临床医学专业-‘‘--J一日lJ吾随着牙髓病学理论和治疗技术的不断发展，根管治疗的成功率已明显提高，但失败的病例仍有发生。目前认为【¨，根管充填后残留在根管内的细菌是根管治疗失败的主要原因。现己证实[2-3l，粪肠球菌(Enterococcusfaec