尘+螨疫苗对过敏性哮喘疗效与机制分析与临床应用观察

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1、重庆医科大学硕士研究生学位论文THEEFFICACYANDMECHANISMTOALLERGICASTHMAWITHMITESALLERGENSVACCINEANDTHEREVIEWoFCLINICALAPPLICATIoNABSTRACTPartITheefficacyandmechanismtoallergicasthmaticmousewithdifferentdoseofdermatophagoidesfarinaevaccineObjectiveToinvestigatetheefficacyandmech

2、anismtOallergicasthmamousewithdifferentdoseofdermatophagoidesfarinae(derf)VaCClne．Methods32femaleC57BL／6micewererandomlydividedinto4groupsaftersettinguptheallergicasthmamodewiththeextractofderf．normalsodiumcontrolgroup(groupA)，lowdosederfgroup(groupB)，middledos

3、ederfgroup(groupC)，lli曲dosederfgroup(groupD)．ThegroupAwastreatedwithnormalsodiumallalong，othergroupsweretreatedintraperitoneallywithcorrespondingdoseder￡Aftertreatment，airway‘’of。was'ed．The,talcel’umbersnd,sitionofreaclavltyofmiceWasassaytotalcellnumbersanOcomp

4、ositionOI●BALFweredetermined．Thephenotypeandtheexpressingofcostimulatorymoleculesofdendriticcellsweredetectedwithflowcytometry．Thelevelsof5重庆医科大学硕士研究生学位论文IL．4，IFN吖inBALFandtheleveloflL-12producedbydendriticcellswerealsomeasuredbyELASA．ResultsTheairwayhyperreact

5、ivity，thematurityofDC，thetotalcellnumberandcompositionofBALFweresignificantreducedingroupCandD，andthereisagreatincreaseoflFN一丫andIL-12ingroupC(P

6、herapeuticdose．Dcplaysagreatroleintheimmunotherapy．Keywords：dermatophagoidesfarinaevaccine；allergicasthma；immunotherapy；dendriticcellPartⅡInfluenceofspecificimmunotherapyondematophagodidespteronyssinusspecificIgG4andpulmonaryfunctioninallergicasthmaObjectiveToi

7、nvestigatethetherapeuticeffect，includingthepulmonaryfunction，airwayreactivity，dermatophagoidespteronyssinus(DerP)specificIgG4，andtheDerPspecificIgE，andthepossible‘anismofandardizedmitesallermmunotheratoallergicmechanismofstandardmedallergenslmmunomerapytOanergt

8、asthmaticchildren．Methods31childrenwithmildtomoderateallergicasthmawere6重庆医科大学硕士研究生学位论文immunotherapywithstandardizedmitesallergensfor25weeks．Thepulmonaryftmction，theDerPspec