strem-1对小鼠肺炎克雷白杆菌肺炎诊断意义的实验性探讨

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1、的相关性，肺组织sTREM．1随IL一6NII增高而增高(P

2、登指数为0．55；当血清CRP水平为2．82ng／ml时，诊断肺炎的敏感度为85％，特异度为75％，约登指数为O．6；sTREM．1联合CRP诊断肺炎的敏感度为95％，特异度为80％，约登指数为0．75。结论：小鼠肺炎克雷自杆菌肺炎肺泡灌洗液、血液、肺组织中sTREM．1表达明显升高，经治疗后表达量均明显下降，提示sTREM．1对细菌性肺炎的诊断有一定的参考价值。关键词：sTREM．1C．反应蛋白肺炎克雷白杆菌肺炎小鼠2ThevalueofsTREM．．1inthediagnosisofbacterialinfectiouspneumoniaillmiceMasterdegreec

3、andidate：QiGuanghongSupervisor：ProfessorWangHuilingVice—Supervisor：ProfessorWangHuilingMajor：InternalMedicineAbstractObiecfive：DeterminetheexpressionlevelsofthesTREM·1inBALF、serumandlungtissue,whichweretakenfromklebsiellarpneumoniamiceandtreatmentedmice，compadngwiththenormalmicetodiscussthemea

4、ningofsTREM一1indiagnosingpneumonia嬲ainflammatorymarker·Methods：Miceselectedrandomlyweredividedintotheregroups，controlgroup、pneumoniagroupandthetreatmentgroup．Eachgroupwas20．Klebsiellarwastaken舶mclinicalinfectionpatients’sputumculture，identifiedbytheVITEK-2system，andit、vaSusedtoestablishthemice

5、pneumoniamodel，whilecontrolgroupWaSadministeredwithanequalvolumeofsaline．CefotaximeWasusedtotreatingthemice，andthedoseWaScalculatedaccordingtheequivalentamountofdrugbetweenaduItandmouse．Theyweretreatmentedforthreedays．Thelungsdidthepathologicalandhistologicalspecimensobservation．IL一6geneexpres

6、singinlungtissueWaSdetectedusingRT．PCRmethod．LevelsofsTREM一1inBALF、serumandthelungtissueweredetectedwithELISAmethodandtheexpressinglevelofCRPinserumwasalsodetectedwithELISAmethod．TheresultswereanalysisedbySPSS15．0．Results：Thelungsofthecontrolgroupwerenormalinthegrossappearance．whilethelungsoft

7、hepneumoniagroupweremarkedlyhemorrhagicandCOngested．TheStandardizationintegralabsorbencyvalue(Nil)ofIL·6inpneumoniagroupWaSsignificantlyhighercomparedwiththecontrolgroup·Thetreatmentgroupcomparingwithpneumoniagroupdeclinedobviously，andt