维甲酸对小鼠b16黑素瘤细胞和正常人黑素细胞的影响

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1、医学系2000级颁士研究生学位论文ABSTRACTThemelanocyteisthepigment-producingdendr廷icceiloftheepidermis．Mela黔eytesresideinthebasallayerinnormaladultepidermisOnemelanocyteisthereforeincontactwithabout36keratinocytes；togethertheyformtheSO．calledepidermalmelaninunit．Melanocytesaremostcommonlyinepid

2、ermisandfollicle．Melanininvolveseumelaninandpheomelanin，it'SsynthesizedthroughaseriesofstagesinwhichtheenzymetyrosinaseactsonmelaninprecursorstOproducethedenselypigmentedgranules。Tyrosinaseisthekeyenzymeofmelanogenesisanddeterminethesynthesisandquantityofmelanin．Becausemelaninis

3、themainpigmentthatdeterminestheskincolor,someresearcherspaymuchattentiontothemodulationofmetanocytesandmelagenesisTherelationshipbetweenmelanocytesandothercells，thecontactbetweenmelanocytesandendocrinesystemorimmunesystem，theeffectofcytokineandthegenefamilyoftyronasewereinvestig

4、atedbasedOilthecultureofmelanoc”esinvitroThemostpracticalstudyistOfindsomedrugswhicharesafeandeffectivetoregulatethemelanocytesandmelagenesis．Retinoidsareusedabroadnowandwithmuchpharmacologicalactivity．TheeffectofitonskincolorisfoundmclinicWeinvestigatedtheeffectsofretinoicacida

5、ndotherdrugsORB16murinemelanomacellsandnormalhumanmelanocytesinvitro，insearchofthemosteffectiveconcentrationandgetinsightOnthemechanismsinvolved．B16murinemelanomacellsandnormalhumanmelanocyteswerecdturedinvitroThecellsfromthesameoriginalbottleweretransplantedtothe96wellcellcultu

6、reclusterThecellconcentrationwereabout5+105／m1．B16murinemelanomacellswereincubatedfor24hours，whilethenormalhumanmelanocyteswereincubatedfor48hours．Thentheculturemediumwasreplacedandretinoicacidorotherdrugs^医学系2000级硕士研究生学位论文wereaddedtoit．Aftertreatingfor72hours，tyrosinaseactivity

7、,melanincontentandeellproliferationrateweredetected．Theresultsshowedthatretinoicacidexhibitedallinhibitoryeffectontyrosinaseactivityandmelaninproductionathighconcentration(>500nmol／L)，andcouldpromotethecellproliferation．Retinoicacidand姆droquinonecouldbecooperativeathigheoncentra

8、tio珏(>500umol／L)．enhancedthedownregulationoftyr