同种异体腱移植重建膝关节交叉韧带的动物实验与临床应用.研究

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1、大载荷和细胞活性均优于一80。C深低温保存组；5、临床应用同种异体腱重建膝关节前、后交叉韧带术后Lysholm评分、物理检查试验、抽屉试验以及患者主观评价均较术前有明显改善。结论1、一80℃深低温保存法和程序冷冻液氮保存法均可有效地用于保存同种异体腱，而程序冷冻液氮保存法更优；2、深低温冷冻保存法处理的同种异体腱移植重建膝关节交叉韧带可以成功地应用于临床。关键词同种异体腱；交叉韧带；重建AbstractObjectiveTostudythehealingprocessofreconstructingthekneecruciateligamentswithallografttendonsthro

2、ughrabbitexperimentandstudythetherapeuticefficacyofclinicalutilization．MethodsTheallograftingwasperformedtoreconstructtheACLofthekneewiththeBPTBofrabbitswhichhadbeenpreservedfor2weekswith一80℃deep—frozenorliquidnitrogendeep—frozenafterprograrmnefreezing，andthecellspoisonresponse，ceilsactivity，themaxi

3、mumloadandmorphologicchangesofthegraftswereobservedtocomparethetwoallograftinggroupseachotherandcomparethemwiththeautograftinggroup．2．AnallografttendonbankwasestablishedforclinicalutilizationtoreconstructthecruciateligamentsofthekneeandtheLysholmScore，physicalexaminationtestandthesubjectiveevaluatio

4、nofpatientswasobservedafteroperating．ResultslAllograftingandautograftingexperiencedthesimilarhealingprocess．Intheearlystage，agreatquantityof7newlybornfibroblastsandbloodcapillarygrewintotheallograftsintrusively，thenthecollagenoftheallograftsnecrosisedandwasreplacedbytheborncollagen．Gradually，itwasre

5、modelledtoanearlynormalstructurewithwell一arrangedcollagenfibers．2．Underthetreatmentofbeingpreservedfor2weekswith一80℃deep—frozenor1iquidnitrogenafterprogrammefreezing，themaximumloadofthepatellartendonhadnoevidentdescent，thecellactivitieswereallwellpreserved，butthegroupwhichwaspreservedbyliquidnitroge

6、nafterprogrammefreezingwasbetter．3．Noevidentrejectreactioncanbeseenduringthehealingprocessafterallografting，andtherejectreactiondeclinedasthetimepassby．4．Threeweeksafertheallografting，therewasnosignificantdifferencebetweenthe2allograftinggroupsinmaximumload，butthecellactivityofthegroupwhichwaspreser

7、vedby1iquidnitrogenafterprogrammefreezingwashigher．Eightweekslater，boththemaximumloadandthecellactivityofthegroupthatwaspreserved8by1iquidnitrogenafterprogrammefreezingwerebetter5．TheLysholmScore，phys