深低温全眼球活性保存角膜的实验.研究

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1、结论：深低温全眼球活性保存角膜方法简便，保存的角膜内皮细胞活性较高，符合临床上穿透性角膜移植的标准。关键词：眼球低温保存角膜内皮细胞酶组织化学ExperimentalResearchonCornealCryoreservationoftheWholeEyeballsPost-graduate：YuJingTutor：ProfessorLanPingMajor：OphthalmologyAbstractBackground：CornealcryopreserVationtechniqueisakindof

2、preservationmethodforcornealgraftestablishedbyCapellain1965，whichcanmakecorneaoccupy“quiescencecondition”．Theviabilityofcornealendothelialcellsdoesnotdereasewiththestoragetime．“Moist—Chamberstorageat’4℃：isasimplewaytOpreservethewholeeyeball，bywhichthegra

3、ftsareeasilytransportedandnoteasytObecontaminated．Buttheviabilityofthecornealendotheliawouldbepreservedforashorttime．We’11combinetheadvantagesofthetWOmethodsabovetOdevelopanewcffectivemethodtOlong—termpreservecornea．Objective：Todevelopthebestmodeofcotnea

4、lcryopreserVationtechniqueandresearchthefactorsthataffectthecornealendotheliumviabilityatlow-temperature．Methods：_Eightyrabbitseyeballsweredividedinto8groupsaccordingtOthemethodsoftheinjectionofDMSOintotheanteriorchamber，thecoolbalancingtime，freezingrate

5、，thethawingcondition．Thestoragetimeforthesecorneaswas30d．10freshrabbiteyeswereasthecontrole．ESRandmorphologyofthecorneaswereevaluatedbytrypan·alizarinredstaining．ThentheelectronmicroscopeexaminationandthehistochemicalstainingofSDHandATPasewerecarriedOU[。

6、andcomparedwiththecontrolegroup．Results：ThebestgroupwiththehighestESRwasselectedwhichtechniqueswereasfollows．theaqueousfluidoftheanteriorchamberwasevacuated，followedbytheinjectionof7．5％(V／V)DMSOandcoolbalanceinrefrigeratorat4℃for30minduringwhichre—inject

7、ionofDMSOwastakentWOtimeS．Thentheeyeballswerestoragedinliquid3nitrogenat一196℃afterrate—freezing30minat一30℃and30minat一80℃successiVely．Theeyeballswerethawedinthewaterbathat40℃．TheESRoftheselectedgroupwas87．53％onaveragewhichwassimilartothatofthecontrolegrou

8、p(98．20％1．ThemeanvalueofIODintheselectedgroupwas133．O士19．2whichwassimilartOthat(134．1土24．0)inthecontrolegrouplightcolor，t=O．1，comparedwiththebestfreezinggroupandthecontrastgroup(P>0．05)．Conclusion：Cornealcryopreservationte