人胃癌与癌旁组织rna氧化损伤水平的比较与临床意义

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1、万方数据温州医科大学硕士学位论文结论：8-oxoGsn在胃癌组织中含量增加，RNA氧化损伤可能在胃癌的发生发展过程中起重要作用。关键词：胃癌；免疫组织化学；8一氧化鸟苷；高效液相一串联质谱4万方数据温州医科大学硕士学位论文LevelandclinicalsignificanceofRNAoxidationinhumangastriccancerandpara—carcinomaAbstractObjective：1．ToinvestigatetheRNAoxidativedamageinhumangastriccancerpara-carcinomatissuesforexploring

2、theroleofRNAoxidationintheofgastriccancer．tissuesandOCCUrrenCe2．ToinvestigatetheRNAoxidativedamageinsignet-ringcellcarcinomaandglandularcancerforexploringtheroleofRNAoxidationinthedifferenttypesofgastriccancer．3．ToinvestigatetheRNAoxidativedamageineachgastriccancerforexploringtheroleofRNAoxidatio

3、ninthedevelopmentofgastriccancer．Methods：1．Thecollectionofhumangastriccancertissuesandpara-carcinomatissues(61cases)：takeacertainamountofintraoperativetumorspecimensputinthevialsimmediately．2．Thelevelof8-oxoGsninthehumangastriccancertissuesandpara—carcinomatissueswerequalitativedetectedbyimmunohi

4、stochemistry．3．TheRNAofhumangastriccancertissuesandpara-carcinomatissueswereextractedbyTRIzoireagent．ThendigesttheextractionRNA．4．Thelevelof8-oxoGsninthehumangastriccancertissuesandpara—carcinomatissueswerequantitativedetectedbyLC—MS／MS．Resuits：1．Theresultsofimmunohistochemicalobservationshowedth

5、at8．OXOGsnwasobviouslyup-regulatedinthegastriccancer．Thepositivestainingmainlyaccumulatedinthecytoplasmofthetumorcells．2．TheODvalueofRNAandtheeleclxophoresisbothdiscoveredthattheextractionRNAhadhighpurityandtheintegritywerebetter．3．Theresultsofmassspectrometryshowedthatthelevelof8-oxoGsninthegast

6、ric万方数据温州医科大学硕士学位论文cancerwashigherthanthatinthepara-carcinomatissues(P<0，05)．Wealsofoundthatthelevelof8-oxoGsninthesignet-ringcellcarcinomawashigherthanthatintheglandularcancer旧<0．05)．Thestudysuggeststhatsignificantdifferencesin8-oxoGsnlevelswereobservedintumor-node—metastasis(TNM)I-IIVS．III-IV(P

7、<0．05)andtheconcentrationof8-oxoGsninIII-IVarehigherthanI—TIConclusion：8-oxoGsnisup-regulatedinthegastriccancer．RNAoxidativedamagemayplayimportantrolesintheoccurrenceofgastriccancer．Keywords：gastriccancer；Immunohistoch