基于fshr表位肽的纳米避孕疫苗的构建与其体内外效应-研究

《基于fshr表位肽的纳米避孕疫苗的构建与其体内外效应-研究》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、万方数据第三军医大学硕士学位论文PreparationofFSHRpeptidenanoVaccineswithdif．fIerentpH—sensitiVitiesandtheiref．fectsonimmunocontraceptionAbstractWiththedeVeIopmentofsocietyandeconomy，theaspirationofmentoparticipateinfamilyplanningisstron91yincreasing．HoweVer，thechoiceofcontraceptionis

2、limitedsofar．Immunocontrac印tiVeVaccineswhichhaVemultipleadvantageshavebeenconsideredoneofthemostpromisingstrategiesinthemalecontraception．FSH／FSHRsignalinginVolVedinactiVatingspennatogenesisandmaintainingnonnalspe珊generation，hasimportantmlesinmalereproduction．FSHRison

3、lyexpressedintestissertolicellsofmale，a芏1dtherefbreitisthebesttargetantigenforcontrac印tiveresearch．Recently，也eresearchoncontraceptiVeVaccinesbasedonFSHRhasachievedexcitingsuccess．TheFSHR32．44peptidewhichwasfiltratedusingthecomputer—basedepitopepredictiontechn0109yoeha

4、sbeenVe“fiedtobe扫【bletoaVoidthesideea’ectsofthereproductiveorgand锄age，andithasanti—fertilityinhibitionef．fects．Currently，therearestillsomeproblemswhich1imititsclinicalapplication，suchasthepoorimmunogenicityofFSHRepitope—basedvaccineinducedlowantibodytiter，thesafetyofF

5、rcund’sadjuvantusedforhuman，theriskofimmuneinjurycausedbyimmunedeViationofthefusionpeptideca玎ier．Nanoca玎iersaresafe，ef艳ctiVe，andtargeteddeliVerysystems．TheyhaveshownuniqueadvantagesindmgdeliVeryandtumorVaccines．Therefore，nanopaniculatevaccinesbasedonFSHRweredeVelopedi

6、nordertosolVeaboVeproblemsofthe印itopevaccine．Inthisresearch，FSHRpeptidethatdoesnotinducepathologicaldamageswasusedasthemodelantigen．Biode伊adalbebiomaterialsincludingpH-sensitiveAc-bCD)andnon．sensitivePLGAwereusedascarriermaterials，toprepareepitopepeptidenanovaccinesof

7、FSHR／Ac—bCDNPandFSHR／PLGANPwithdiHbrentpH—sensitiVities，respectiVely．TheexperimentswerecarriedouttoinVestigatetheefrectsofimmuneresponsesandanti—fertilitywiththesenanoVaccinesinVitroandinViVo．ThestudiescanbediVidedintothefollowingthreesections．1．Fabricationandcharacte

8、rizationofFSHRnanovaccinewithdif．ferentpH一4万方数据第三军医大学硕士学位论文senSltlvltles．Earlier，thedominant如nctionalepitopepeptide(FSHR32_4