sip蛋白高亲和性噬菌体介导的牛源无乳链球菌间接elisa方法的建立

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1、万方数据CONTENTSAbstractinChinese⋯⋯⋯⋯⋯⋯⋯⋯．⋯⋯⋯⋯．．⋯⋯．．．⋯⋯⋯⋯⋯．⋯．⋯⋯．⋯⋯⋯⋯⋯⋯⋯⋯⋯．．．⋯⋯．⋯．．．．．．．IAbstractinEnglish．⋯⋯⋯⋯⋯⋯⋯⋯．⋯．．．．．．．．．．．．．．⋯．．．．．．．．．．．．．．．．．．．⋯．．．．．．⋯．⋯⋯．⋯．⋯．⋯．．．．．．．．．．．．．．．．⋯．．．．．III1Introduction．⋯．．．．⋯⋯⋯⋯⋯⋯⋯⋯．⋯⋯⋯⋯．．．⋯⋯⋯⋯⋯．．⋯⋯．．．．．⋯．⋯⋯⋯．⋯⋯⋯⋯．⋯⋯⋯．．．⋯⋯⋯．⋯

2、．I1．IThesummarizeofCOWmastitis．⋯．⋯．⋯⋯．．⋯⋯⋯．．⋯．⋯⋯⋯⋯⋯．．．．．⋯．⋯．⋯⋯．．⋯⋯⋯⋯．．．．11．2TheresearchdevelopmentofStreptococcusagalactiae⋯⋯⋯．．．．．．．⋯．⋯⋯⋯⋯⋯⋯．．．．．．．．．．2I．2．1ThebriefofStreptococcusagalactiae⋯⋯⋯⋯⋯．⋯．⋯．．．．．⋯⋯⋯．．⋯⋯．．．⋯．．．．．．⋯⋯⋯．21．2．2TheresearchdevelopmentofStre

3、ptococcusagalactiaesurfaceproteins．．．．．．．．．．．．．⋯21．2．3Researchprogressofvirulencefactors⋯⋯⋯⋯．．．⋯．．．．．．．．⋯⋯⋯⋯⋯．．．．．⋯．⋯⋯⋯．．．．．．．51．2．4DetectionmethodofStreptococcusagalactiae．。。。⋯．。．．．．。．．．．．．．。．。。。，，⋯．．．。．。。⋯。⋯。。。．。。。．．71．3Researchprogressofphagedisplaytechnol

4、ogy⋯．⋯．⋯⋯．．．⋯．⋯⋯．⋯．⋯⋯．．．．．．⋯．⋯⋯⋯．．．81．3．IPrincipleandtypesofphagedisplaytechnology．．．．．．．．．．．．．．．．．⋯．．．．．．．．．．．．．⋯．．．⋯．⋯．．．．．．91．3．2Applicationofphagedisplaytechnology．⋯．．．．．．⋯．⋯⋯．．⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．101．4Thepurposeandsignificanceofexperiment．⋯．．⋯．．．．⋯⋯⋯．．．⋯⋯⋯．．．．

5、⋯⋯⋯．．⋯⋯⋯⋯．．112MaterialsandMethods．．．．⋯．．．．．．．．．．．．．．⋯⋯．⋯⋯．⋯⋯⋯⋯⋯⋯．．⋯．．．⋯⋯．．．⋯⋯⋯．⋯．．．．．．．．⋯．⋯⋯⋯⋯．．122．IMaterial⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯122．1．1Bacterialstrainandvector⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．122．1．2Toolenzymesandreagents⋯⋯．⋯⋯⋯⋯⋯⋯⋯．⋯⋯⋯⋯．．⋯⋯⋯⋯⋯⋯

6、⋯．．．⋯．⋯⋯．．．．122．2Experimentalmethods⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯122．2．1Primerdesign⋯．⋯⋯．⋯．．．．．．⋯⋯⋯⋯．⋯⋯．⋯⋯⋯⋯⋯⋯．⋯．．⋯．．．．．．．．．⋯⋯⋯⋯．．．．．⋯⋯．．．122．2．2Plasmidprep．⋯．．⋯．．．．⋯．⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．⋯⋯⋯．．⋯⋯⋯．．．．⋯⋯⋯．．．．122．2．3Amplificationofthepurposegene⋯⋯⋯⋯⋯．⋯．⋯⋯⋯⋯⋯⋯．⋯．

7、．⋯⋯⋯⋯⋯．⋯⋯．．．．122．2．4Identificationofrecombinantplasmid⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．132．2．5PorteinexpressionofSip—pGEX一6P一1．⋯⋯．．⋯．．．⋯⋯．⋯⋯⋯⋯⋯．．．．．．．．．．．．．．．．．⋯⋯．．．132．2．6Porteinpurification⋯⋯。⋯⋯⋯⋯⋯⋯⋯⋯⋯．⋯．．．．。⋯。。。．．⋯．⋯⋯⋯⋯⋯。．。．。。。。．．。。。．。．。⋯。．。132．2．7Porteinrenaturation

8、⋯⋯⋯⋯．．．⋯⋯⋯．⋯⋯⋯⋯⋯⋯．⋯．⋯⋯⋯．．⋯⋯．．．．．．．．．．．．．．．．．．⋯⋯．．．132．2．8Panningofphage⋯⋯⋯．⋯⋯⋯⋯⋯⋯⋯．．．．．⋯．．．．．．．．．．．．．．．．．．．⋯⋯⋯．．．．⋯．．．．．．．．．．．．．⋯．⋯．．142．2．9Amplificationandthedropdegreedeterminationofpha