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1、不同复苏措施对猪心肺复苏后心肌凋亡影响【摘要】目的本研究探讨猪心肺复苏后的心肌功能障碍是否有凋亡机制的参与以及心肺复苏的策略选择对于凋亡是否有影响。方法22头五指山小型猪被随机(随机数字法)分为假手术组(SHAM)(6只)、先除颤组(DF)和先按压组(CF)(各8只)。建立室颤8min小型猪模型，监测各组的血流动力学指标，用Westernblot法检测心肌组织Bel-2、Bax和caspase-3的蛋白表达，用实时荧光PCR法定量caspase-3的mRNA水平，TUNEL法检测心肌组织凋亡。应用SPSS17.0统计软件进行数据处理，组间比较采用成组t检验，多组均数比较用方差分析。以PO.
2、05)o结论凋亡机制可能参与了心肺复苏后猪心肌功能障碍,但心肺复苏的策略选择对于凋亡的影响并不大。【关键词】凋亡；心肺复苏；复苏后心肌功能障碍;Caspase-3；Bcl-2/BaxEffectsofdifferentresuscitationmodalitiesonpost-resuscitationmyocardialapoptosisinporcinemodelsofcardiacarrestGUWei,LIChun-sheng,YINWen-peng,HOUXiao-min,GUOZhi-jun,ZHANGDa,WUCai-jun.EmergencyDepartment,Beiji
3、ngChaoyangHospital,CapitalMedicalUniversity,Beijing100020,ChinaCorrespondingauthor:LIChun-sheng,Email:lcscyyy@163・com【Abstract】ObjectiveToconfirmwhetherinpost-resuscitationmyocardialdysfunctioninvolvedinmyocyteapoptosismechanisminporcinemodelofcardiacarrestandapoptosisindexvariedfromdifferentmodal
4、itiesofcardiopulmonaryresuscitationornot・MethodsAtotalof22WZSPinbredsmallswinewererandomly(randomnumber)dividedintoshamoperationgroup(SHAM)(n=6),defibrillationfirstgroup(DF,n=8)andchestcompressionfirstgroup(CF,n=8).Eightminutesafterventricularfibrillationwassetup,standardCPRwascarriedoutsubsequent
5、lyafterdefibrillationinporcinemodelsofcardiacarrestinDFgroupanddefibrillationafterstandardCPRinCFgroup,andhemodynamicsweremonitored.Twenty-fourhoursafterrestorationofspontaneouscirculation(ROSC),animalsweresacrificed,andmyocardialspecimenswereexaminedwithelectronmicroscopy,Westernblot,quantitative
6、RT-PCR,andtermina.1deoxynucleotidyltransferase-mediateddUTPnickendlabeling(TUNEL)assay.TheexperimentaldatawereanalyzedbySPSS17.0.Studentsttestwasemployedforcomparisonsbetweentwogroups・DifferenceswithingroupsatdifferenttimeintervalswerecomparedwithrepeatedmeasuresANOVA.Atwo-tailedvalueofP0.05)・Conc
7、lusionsCaspase-3-mediatedapoptosismightbeoneofthemainpathologicalmechanismsofpostresuscitationmyocardialinjuryinaporcinemodelofcardiacarrest,buttherewasnostatisticallysignificantdifferenceinapoptosisindexbetweent
