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1、XX大学毕业论文弥漫性大B细胞淋巴瘤bcl姓名：2014年6月25日弥漫性大B细胞淋巴瘤bcl作者：蒋会勇，陈愉，张三泉，朱梅刚，赵彤【关键词】弥漫Correlationbetweenbcl2/IgHgenerearrangementandGCBmolecularsubtypeindiffuselargeBcelllymphoma[Abstract]AIM:Toexplorethecorrelationbetweenbcl2/IgHgenerearrangementandgerminalcenterBcel
2、llike(GCB)molecularsubtypeindiffuselargeBcelllymphoma(DLBCL).METHODS:SelfdesignedheminestedPCRwasusedtodetectbcl2/IgHgenerearrangementin60casesofDLBCL・PositivePCRproductswereclonedandsequenced.Thetissuemicroarrayof60specimensofDLBCLwaspreparedandtheexpress
3、ionsofCD20,CD10,Bcl6andMUM1wereinvestigatedbyimmunohistochemicalSPmethod.GCBandnongerminalcenterBcelllike(nonGCB)weresubclassified.RESULTS:Sixofthe60DLBCLwerebcl2/IgHpositivebyconventionalPCRmethod・SelfdesignedheminestedPCRwasusedtoamplify6positivecasesaga
4、inandbcl2/lgHgenewasverifiedrearrangementin5casesbycloningandsequencingand1hadthegenesegmentofBAC331191ofhumanchromosome19.CD20waspositiveinthe60DLBCLcases-PositiveexpressionratesofCD10,Bcl6andMUM1were43.3%,46.7%and61.7%respectively.32(53.3%)wereGCBand28(4
5、6.7%)werenonGCB.Bcl2/IgHgenerearrangementpositivecaseswereallGCBsubtypes,whichweresignificantlycorrelatedwiththeexpressionofCD10(P=0.012)andnotwithBcl6andMUM1.CONCLUSION:SelfdesignedheminestedPCRcanimprovethedetectionaccuracyofbcl2/IgHgenerearrangement.Bcl
6、2/IgHgenerearrangementdetectionhelpstheDLBCLdeterminationofGCBsubtypes・【Keywords]lymphoma,Bcell,diffuselarge；bcl2;generearrangement【摘要】目的：探讨弥漫性大B细胞淋巴瘤(DLBCL)bcl2/IgH基因重排与分子亚型生发中心样(GCB)的相关性.方法:采用自行设计的半巢式PCR对60例DLBCL的bcl2/IgH基因重排进行了检测，并对阳性产物进行克隆、测序.同时采用免疫组化S
7、P法在组织微阵列上同步观测CD20,CD10,Bcl6,黑色素瘤相关抗原(突变体)1(MUM1)的表达，进行GCB和非生发中心样(nonGCB)分子分类.结果：经常规法扩增bcl2/IgH,有6/60例DLBCLbcl2/IgH阳性；在6例阳性样本中，采用本组设计的半巢式PCR扩增，经克隆及测序证实5例bcl2/IgH基因重排阳性，1例为人类第19号染色体BAC331191基因的片段.60例DLBCLCD20表达全部阳性；CD10,Bcl6,MUM1的阳性表达率分别为43.3%,46.7%,61.7%；GC
8、B32(53.3%)例，nonGCB28(46.7%)例.bcl2/IgH基因重排阳性的病例均属GCB分子亚型，且与CD10表达有显著性相关(P=0.012),而与Bcl6及MUM1表达无相关性.结论:使用本组设计的半巢式PCR可提高bcl2/IgH基因重排检测的准确性.bcl2/IgH基因重排的检测可协助确定部分GCB分子亚型的DLBCL.【关键词】弥漫性大B细胞淋巴瘤；bcl2;基因重排0引言弥漫性大B细胞淋
