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1、培养大鼠脑皮层神经元损伤后c-fos蛋白表达Thec-fosexpressionwasdevelopedaftertheinjuryofneuronsinthebraincortexUpdatedate:2010-09-08kingofChina,zhupeifangofthestateofzhupeifang【abstract】objectivetoobservetheroleofc-fosinthesecondarybraininjuryaftertheinjuryofneuronsinrats・Methodsthecortexneuronsofthe10to12daySDm
2、iceweredamagedbyglutamateandmechanicalseratch.UsingSP,thedoublemarkingofimmunisationwascarriedout・Asaresult,twohoursafterglutamate,c-fosbegantobeexpressed,andinfourhourstheywereclearlyconcentrstedinthenucleus・Thetwohoursafterthetraumahadbeenshowninthedirectlesionontheedgeofthechannel,thec-fos
3、wereexpressed,andtheneuronsthatwerefarawayfromthedirectdamageareawereshownfourhoursafterthetrauma・Thesmallnumberofneuronsprotrudingfromthedistalendoftheneuron,andthec-fosparticle;Sixhoursaftertheinjury,theneuronsbegantodisintegrate,butthec-fosparticlesdidnotdisappear・Conclusionglutamateinjury
4、cancauseculturedneuronsc-fosproteinexpressionofculturedcorticalneuronsandglialcellsafterinjurycanreleasetoxicsubstances,trainingafterinjuryofneurons一cfosproteinexpressionmaybeassociatedwithapoptosis・【keywords】c-fosneuronsdevelopglutamatesecondarybraininjuryTheExpress!onofc-fosAfterCulturedCor
5、ticalNeuronInjuryinRatsWANGke-wan,YANGZhi-huan,WANGzheng-guo,etal.ResearchInstituteofSurgery,ThirdMilitaryMedicalUniversity,Chongqing400042[Abstract]AimToobservetheexpressionofc-fosproteininculturedcorticalneuronssubjecttoeitherglutamateormechanicalinjuryinrats,andtostudytheeffectofc-fosprote
6、inonbraininjury.MethodsMixedculturedcorticalneuronsfromSDratswereinjuredbyeitherglutamateormechanicalforce,thentheexpressionofc-fosproteinwasstudiedwithdouble-labeledimmunohistochemistry.ResultsTwohoursafterglutamateinjury,theexpressionofc-fosproteinbeganinthenucleiand4hourslateritreachedpeak
7、,andc-fosproteinonlyexpressedinthedirectinjuredareaattheedgeoftraumatictractsafter2hoursandbegantoexpresstill4hoursintheindirectinjuredareafarfromtraumatictracts・Thepositiveimmunoreactionofc-fosproteingranuleremained・ConclusionThec-fosprotein
