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1、活性维生素D对2型糖尿病大鼠肾组织胰岛素信号通路PI3K/AKT的影响邓娟娟张丹韩晴孔亚婷朱慧铭朱正庭叶明彭艳潘希峰李书国（三峡大学第一临床医学院，湖北宜昌市中心人民医院老年病科，湖北宜昌443003）【摘要】目的探讨活性维生素D对2型糖尿病大鼠肾组织胰岛素相关信号分子胰岛素受体（IRβ）、磷脂酰肌醇3激酶(PI3K)、蛋白激酶B(Akt／PKB)表达的影响。方法30只雄性Wistar大鼠随机分为普通饲料组即正常组（N组10只）和高脂高糖饲料组（20只），高脂高糖饲料组以小剂量(30mg/kg)链脲佐菌素（STZ）加高糖高脂饮食诱导2型糖尿病模型后随机分为2组，分别经腹腔注射2.5ug/kg
2、.d-1花生油（M组,10只）或腹腔注射2.5ug/kg.d-1溶于花生油中的活性维生素D（D组，10只)。8周后留取大鼠血标本，测定血糖、血肌酐及尿素氮,处死大鼠留取肾组织。WesternBlot检测肾组织中胰岛素相关信号分子IRβ、PI3K、Akt的表达及其磷酸化的水平。结果糖尿病组大鼠血糖、尿素氮、肌酐高于正常（P<0.05)）；而相对于糖尿病组，维生素D干预组血糖、尿素氮及肌酐明显降低（P<0.05）.WesternBlot显示相较于正常组，糖尿病组大鼠肾组织中三组总的IRβ、Akt的表达无明显改变而其磷酸化水平及PI3K的水平明显下调，而维生素D干预后明显上调(P<0.05).结论
3、 活性维生素D可激活胰岛素信号通路PI3K/AKT的表达，对糖尿病大鼠肾脏损伤发挥保护作用。【关键词】1,25-二羟胆骨化醇；糖尿病；PI3K/AKTTheimpactof1,25-dihydroxyvitaminD3oninsulinsignalpathaboutPI3K/AKTonkidneytissueoftype2diabeticratsDENGJuan-juan;ZHANGDan;HANQin;KONGYa-ting;ZHUHui-ming;ZHUZheng-ting;YEMing;PENGYan;PANXi-feng；LIShu-guoDepartmentofGeriatrics
4、,FirstCollegeofClinicalMedicalScience,ChinaThreeGorgesUniversity,CentralPeople'sHospitalofYichang,Yichang443003,Hubei,China【Abstract】ObjectiveTostudytheimpactof1,25-dihydroxyvitaminD3oninsulinsignalpathaboutPI3K/AKTonkidneytissueoftype2diabeticrats.Methods30wistarratsweredividedintonormal(10rats)an
5、dexperimentalgroupsrandomly(20rats).Wistarratsofexperimentalgroupwereinjectedlowdosestreptozotocin(25mg/kg)andwerefedwithdietsenrichedinbothglucoseandfattoformtype2diabeticmodel,thenweredividedinto2groupsrandomly.Andeachgrouphad10diabeticrats.1,25-dihydroxyvitaminD3(VitaminDgroup)orpeanutoilinthesa
6、medoseas1,25-dihydroxyvitaminD3(Diabeticgroup)wasinjectedintodiabeticratsthroughenterocoelia,thenkilledallratsafter8weeks.Thelevelsofbloodsugar(BS),bioodureanitrogen(BUN)andserumcreatinine(Scr)weremeasured.TheexpressionofP-IR,P-AKTandPI3KweredetectedbyWesternblot.Results.Comparedthenormalgroup,Thel
7、evelsofBS,BUNandScrwereincreased,WhereasthoseweredecreasedintheVitaminDgroup.TheresultofwesternblottingshowedthattheexpressionofP-IR,P-AKTandPI3KinkidneyofDiabeticgroupreducedsignificantlycomparedwithnormal
