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1、雌激素动员EPCs修复损伤血管内皮研究赵晓辉1，吴楠2，尹扬光1，方玉强3，陈剑飞1，崔斌1，黄岚1(400037重庆，第三军医大学新桥医院心血管内科1；400038重庆，第三军医大学西南医院眼科2；400042重庆，第三军医大学大坪医院野战外科研究所心血管内科3)[摘要]目的观察雌二醇（Estradiol，E2）对小鼠外周血内皮祖细胞(endothlialprogenitorcells，EPCs)动员及损伤颈动脉内皮修复的效果。方法小鼠去势后给予雌激素治疗，随后建立小鼠颈动脉损伤模型，分别于1天及3天后流式细
2、胞分析小鼠外周血EPCs数量变化，Evens兰染色评价7天后颈动脉再内皮化面积。结果颈动脉损伤前各组外周血EPCs数量分别为卵巢切除＋雌激素组(0.064±0.016％)、卵巢切除组(0.028±0.01％)及未切除卵巢组(0.063±0.022％)(n=11)。颈动脉损伤后1，3天各组外周血EPCs数量分别为，卵巢切除＋雌激素组(0.42±0.135％，n=6；1.47±0.38％，n=5)、卵巢切除组(0.13±0.024％，n=6；0.25±0.024％，n=6)、未切除卵巢组(0.43±0.16％，n=
3、6；0.65±0.21％，n=4)。颈动脉损伤7天后卵巢切除组再内皮化面积较未切除卵巢组及卵巢切除＋雌激素组减少(28.33±13.49%，n=5；69.53±14.14%，n=4；83.11±7.94%，n=5；P<0.01)；结论雌二醇可动员小鼠外周血EPCs，修复损伤血管内皮。[关键词]雌二醇；内皮祖细胞；动员；内皮损伤；小鼠中图分类号：Q579．1文献标识码：AEstrogenInducesRecoveryofInjuredArteryEndotheliumbymobilizingEndothelial
4、ProgenitorCellXiaohuiZhao,YangguangYin,YuqiangFang,JianfeiChen,BincuiandLanHuang*CardiovascularDiseaseResearchCenter,XinqiaoHospital,ThirdMilitaryMedicalUniversity,Chongqing400037,China*Correspondence:ProfessorLanHuang;e-mail:huanglan260@yahoo.com.cn.Abstrac
5、t:Introduction:Mobilizationofendothelialprogenitorcells(EPC)restoresendothelialfunction,representinganoveltherapeuticdirectionforinjuredbloodvesselrecovery.ThepresentstudywasdesignedtodeterminetheeffectofestrogenonEPCmobilizationandregenerationofendotheliumi
6、nmice.Methods:Variectomyisperformedbeforetreatmentof17β-estradiol.Then,carotidarteryinjurywaspreformedandneointimawasevaluatedbyH.Estaining.Oneandthreedayslater,mobilizationofEPCswasevaluatedbyFACSasdoublepositiveofSca-1/VEGFR-2.Evensbluewasinjectedandareaof
7、reendothelizationwascalculatedafter7days.Results:Oneorthreedaysaftercarotidarteryinjury,EPCsofperipheralbloodwere0.42±0.135%(n=6),1.47±0.38%(n=5)(ovariectomy＋E2);0.13±0.024%(n=6),0.25±0.024%(n=6)(ovariectomy);0.43±0.16%(n=6),0.65±0.21%(n=4)(non-ovariectomy);
8、0.12±0.019%(n=6),0.25±0.062%(n=6).Areaofre-endothelizationwere(ovariectomy,28.33±13.49%,n=5)vs(ovariectomy＋E2,69.53±14.14%,n=5)vs(non-ovariectomy,83.11±7.94%,n=4)(P<0.01)..Conclusion:Estrogencan
