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1、泪液高渗透压导致的小鼠干眼模型的建立李晶，付川，谢汉平（第三军医大学西南医院/西南眼科医院重庆400038）[摘要]目的制作一种新的泪液高渗透压导致的小鼠干眼模型。方法60只雌性6～8周龄BALB/c鼠，随机具体是什么随机分组方法？分为空白组、对照组和实验组。对照组采用308mOsmol/L氯化钠溶液点眼，每天5次，实验组采用500mOsmol/L氯化钠溶液点眼，每天5次，空白组不点眼。分别在第0，7，14，28，42天行Schirmer实验、角膜荧光素钠染色和评分、眼表虎红染色观察、泪液蕨类实验、角膜上
2、皮HE染色观察和角膜上皮厚度测量、结膜上皮PAS染色观察和杯状细胞计数。第42天行角膜上皮扫描电子显微镜观察。结果应补充主要结果的数据和统计值。在第0天各组间无统计学差异。第7天起，与空白组和对照组相比，实验组BALB/c鼠出现泪液生成和分泌减少，角膜荧光素钠染色和虎红染色出现点、片状着色，角膜荧光素钠染色评分升高，泪液蕨类图形减少。第14天起，结膜杯状细胞计数减少。角膜上皮基底[基金项目][通信作者]谢汉平，电话：(023)68765377，E-mail:xiehanping@mail.tmmu.com
3、.cn部细胞排列紊乱缺失，角膜上皮分层减少，出现空泡，角膜上皮层厚度减少，角膜上皮细胞微绒毛丢失。结论泪液高渗透压可以在小鼠眼表引起类似干眼的病理损害，泪液高渗透压在干眼的发病中起重要作用。[关键词]干眼；泪液；高渗透压[中图法分类号][文献标志码]AAMurineModelofDryEyeInducedbyHyperosmolarStressinTearsJingLi,FuChuan,HanpingXie(SouthwestHospital,SouthwestEyeHospital,ThirdMilit
4、aryMedicalUniversity,Chongqing,China400038)[Abstract]ObjectiveToestablishanovelmurinemodelofdryeyeusingHyperosmolarsaline.MethodssixtyfemaleBALB/cmiceaged6to8weekswererandomlydividedintoblankgroup,controlgroupandexperimentalgroup.Themiceincontrolgroupwere
5、treatedwith308mOsmol/Lsodiumchloridesolution,fivetimesperday.Themiceinexperimentalgroupweretreatedwith500mOsmol/Lsodiumchloridesolution,fivetimesperday.Schirmertest,fluoresceinstaining,cornealscoring,rosebengalstaining,tearfernsexperiments,cornealepitheli
6、alHEstainingandthicknessmeasuring,conjunctivalepithelialPASstainingandgobletcellcountingwereconductedonday0,7,14,28,42.Cornealsurfacewereobservedbyscanningelectronmicroscopyonday42.Results应补充主要结果的数据和统计值。Nostatisticallysignificantdifferencesbetweenthegroup
7、swerefoundatbaseline.Decreasedtearsecretionandtearfernsorincreasedcornealfluoresceinandrosebengalstainingweresignificantlypresentonday7,14,28and42inmiceinexperimentalgroup.Gobletcelldensitywassignificantlydecreasedonday14,28and42.Thecorneaofmiceinexperime
8、ntalgroupwerefoundabnormalityofarrangementofthebasalcells,decreasedthicknessofepitheliallayer,presentationofvacuole,lossofmicrovilli.ConclusionThelesionsonthemurineocularsurfaceinducedbyhyperosmolarstressaresimilart
