ngal和mmp-9在卵巢上皮性癌中的表达及相关性研究

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1、NGAL和MMP-9在卵巢上皮性癌中的表达及相关性研究郭艳，苑中甫★郑州大学第一附属医院妇产科　河南郑州　450052★通讯作者:教授，硕士生导师,E-mail:yzf236@163.com【摘要】目的：检测中性粒细胞明胶酶相关载脂蛋白(neutrophilgelatinase-associatedlipocalin,NGAL)和基质金属蛋白-9（matrixmetalloproteinase-9,MMP-9）在卵巢上皮性肿瘤组织和血清中的表达，了解两者与卵巢癌临床病理特征的关系。方法：应用半定量逆转录一聚合酶链反应法（RT-PCR）和酶联免疫吸附

2、双抗体夹心法（ELISA）检测50例上皮性卵巢癌、21例卵巢良性肿瘤和18例正常人组织和血清中NGAL和MMP-9的表达水平。结果：①卵巢癌患者的组织及血清中NGAL和MMP-9表达明显高于卵巢良性肿瘤组和正常对照组(P<0.05)；二者的高表达与临床分期、有无淋巴结转移正相关(P<0.05)；卵巢癌患者的组织及血清中NGAL与卵巢癌的组织分化程度正相关，MMP-9与卵巢癌的组织分化程度负相关。②NGAL与MMP-9在卵巢癌患者的组织及血清中表达均呈正相关(r=0.740，r=0.676，P<0.05)。结论：NGAL和MMP-9的表达上调与卵巢上

3、皮性癌的恶性生物学行为关系密切,二者在卵巢癌的发生、侵袭及转移中具有协同作用。【关键词】卵巢肿瘤;中性粒细胞明胶酶相关载脂蛋白;基质金属蛋白酶-9【中图分类号】R737.31【文献标志码】:AStudyofexpressionandcorrelationofNGALandMMP-9inepithelialovariancancers　GuoYan,YuanZhongfu.DepartmentofObstetricsandGynecology,TheFirstAffiliatedHospitalofZhengzhouUniversity,Henan,

4、Zhengzhou,450052,China【Abstract】Aim:ThisstudywastoinvestigatetheexpressionofissueorserumlevelsofNGALandMMP-9inepithelialovariantumors,andexploretheircorrelationwithclinicopathologicalcharacteristics.Methods:TheexpressionofNGALandMMP-9inserumandovariantissueweredetectedin50pati

5、entswithepithelialovariancancers,21caseswithovarianbenigntumorsand18healthywomenbyReversetranscriptase-polymerasechainreaction(RT-PCR)andELISA.Results:①ThelevelsofNGALandMMP-9inserumandovariantissueofpatientswithepithelialovariancarcinomaswerehigherthanthoseofpatientswithovari

6、anbenigntumorandthecontrolgroup(P<0.05).TheOverexpressionofNGALandMMP-9werecorrelatedsignificantlywithclinicalstage,metastasisoftheovariancancer(P<0.05).Moreover,inserumandovariantissueofpatientswithepithelialovariancancers，theexpressionofNGALhadmarkedpositivecorrelationwithhi

7、stologicaldifferentiation(P<0.05)andtheexpressionlevelofMMP-9wasnegativecorrelatedwithhistologicaldifferentiation(P<0.05).②ThepositivecorrelationwasobservedbetweentheexpressionofNGALandMMP-9(r=0.740，r=0.676，P<0.05)inserumandovariantissueofpatientswithepithelialovariancancers.C

8、onclusion:Theseresultssuggestedthatup-regulatedexpressionofNG