资源描述：
《cxcr4sdf-1对鼻咽癌细胞增殖与迁移能力的影响》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。
1、CXCR4/SDF-1对鼻咽癌细胞增殖与迁移能力的影响邱际华刘求真；陈丽；焦锋；姚开泰(南方医科大学肿瘤研究所，教育部和广东省共建人类重大疾病转录组学和蛋白质组学重点实验室,广东省分子肿瘤病理学重点实验室,广东广州510515)提要：目的观察鼻咽癌不同恶性程度的细胞中趋化因子受体CXCR4的表达,检测SDF-1/CXCR4反应轴对鼻咽癌细胞的增殖作用,SDF-1对CXCR4阳性肿瘤细胞的趋化作用,探讨CXCR4/SDF-1生物学轴对人鼻咽癌细胞增殖与迁移能力的影响.方法以鼻咽癌成瘤高转移细胞株5-8F及成瘤不转移细胞株6-10B为研究对象，采用We
2、sternBlot免疫印迹法检测鼻咽癌细胞株CXCR4蛋白的表达情况，SDF-1及CXCR4阻断剂AMD3100作用于两种细胞后,MTT法检测细胞的增殖能力,体外迁徙实验检测SDF-1/CXCR4反应轴对鼻咽癌细胞的趋化作用。采用SPSS13.0软件包进行ANOVA方差分析和t检验。结果5-8F细胞株CXCR4蛋白的表达明显高于6-10B细胞株；SDF-1能明显增强5-8F细胞增殖与迁移能力，CXCR4阻断剂AMD3100作用后,5-8F细胞增殖与迁移能力明显降低；SDF-1对6-10B细胞的迁移及增殖能力无明显影响。结论CXCR4/SDF-1生物
3、学轴与鼻咽癌细胞株的增殖与迁移有一定的关系，AMD3100可明显抑制鼻咽癌细胞的增殖与迁移能力.关键词：鼻咽癌;增殖；迁移；CXCR4；SDF-1EffectofCXCR4/SDF-1onproliferationandmigrationofHumanNasopharyngealCarcinomacellQIUJi-hua，LIUQiu-zhen，CHENLi,JIAOFeng,YAOKai-tai(InstituteofCancerResearch,KeyLaboratoryforTranscriptomicsandProteomicsofHum
4、anFatalDiseasesSupportedbyMinistryofEducationandGuangdongProvince,KeyLaboratoryforMolecularTumorPathologySupportedbyGuangdongProvince,FacultyofBasicMedicine,SouthernMedicalUniversity,Guangzhou510515)Abstract：ObjectiveThisstudywasaimedtoevaluatetheexpressionofchemokinereceptors
5、CXCR4inNPCcellslineandtodeterminetheroleofCXCR4/SDF-1intumorcellproliferationandmigration.MethodsTheexpressionofCXCR4proteininHumanNasopharyngealCarcinomacellswasmeasuredbyWesternBlotanalysis.AftertheadditionofSDF-1andAMD3100,thechangesofcellgrowthwereobservedbyMTT,Thechangeso
6、fcellmigrationweremeasuredbyTranswellinvitro.ResultsWesternBlotshowedthat5-8Fcellsline,whichtumorigenicandhighmetastaticability,expresshigherCXCR4levelsthan6-10Bcellline,tumorigenicandlackingmetastaticability.thegrowthrateandthenumberofmigrationcellin5-8FcellsincreasedbySDF-1,
7、andsignificantlydecreasedinvitroaftertheadditionofAMD3100,while6-10BcellswerenotinrespondtoSDF-1.ConclusionCXCR4/SDF-1mayplayanimportantroleinNPCcellsproliferationandmigration,AMD3100canefficientlysuppressitsfunction..Keywords:NasopharyngealCarcinoma;proliferation;migration;CX
8、CR4;SDF-1鼻咽癌是我国南方及东南亚地区常见的一种恶性肿瘤，尤以珠江三角洲一带多见，具有“广东瘤”之称。由于鼻咽部淋
