ova致敏小鼠肠道黏膜muc1,muc2表达研究

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1、OVA致敏小鼠肠道黏膜MUC1、MUC2表达研究通信作者：黎海芪，Email：haiqili2010@hotmail.com谢芸芸1黎海芪21江西省儿童医院儿童保健科（江西330006）；2儿童发育疾病研究教育部重点实验室；儿科学重庆市重点实验室；重庆市儿童发育重大疾病诊治与预防国际科技合作基地；重庆医科大学附属儿童医院儿童保健科（重庆400014）[摘要]目的了解MUC1和MUC2在OVA致敏小鼠肠道中的表达情况。方法16只5~6周雌性BALB/c小鼠随机分为实验组及正常组，建立OVA致敏小鼠模型，ELISA法检

2、测血清OVA特异性IgE水平，AB/PAS特殊染色法检测胃肠道黏蛋白表达及采用免疫组化SP法检测胃肠道MUC1及MUC2的表达。结果OVA致敏小鼠血清OVA特异性IgE水平较正常组升高;肠道AB/PAS染色示杯状细胞数量增加及形态变化、黏蛋白分泌增加;正常组小鼠空肠MUC1不表达、MUC2阳性表达，而OVA致敏小鼠肠道MUC1阳性表达，且MUC2高表达（P<0.05）。结论MUC1与MUC2在OVA致敏小鼠有异常表达，与疾病发生发展的关系有待进一步研究。[关键词]食物过敏；黏蛋白；免疫组化SP法；Expressio

3、nofMUC1andMUC2intheOVA-sensitizedmiceXIEYun-yun1,LIHai-qi2(1DepartmentofPrimaryChildCare,JiangXiChild-ren’sHospital;2MinistryofEducationKeyLaboratoryofChildDevelopme-ntandDisorders,Key Laboratory of Pediatrics in Chongqing,CSTC2009CA5002,Chongqing Internationa

4、l Science and Technology Cooperation Center for Ch-ild Development and Disorders,DepartmentofPrimaryChildCare,Children’sHospitalofChongqingMedicalUniversityChongqing,400014,China)[Abstract]ObjectiveToexploretheexpressionsofMUC1andMUC2intheOVA-sensitizedmice.Me

5、thods16femaleBALB/cmicewith5~6weekswererandomlydividedintohypersensitivityandnormalgroups.TheanimalmodeloffoodallergywasestablishedwithOVAinhypersensitivitygroup,thentheOVAspecificIgEleversweremeasuredbyELISA;Mucinsinstomachandjejunumwererespectivelyobservedby

6、AB/PAS;Immuno-histochemicalSP-stainingmethodwasperformedtodetecttheexpressionsofMUC1andMUC2instomachandjejunum.ResultsTheOVAspecificIgEleverswereincreasedinOVA-sensitizedmice,andthegoblelcellsandmucinswereincreasedinjejunum.TheMUC1injejunumwereexpressedinOVAse

7、nsitizedmice,butnotinnormalmice;thentheMUC2injejunumoftwogroupswereexpressed,butitwassignificantlyhigherinOVAsensitizedmicethanthatinnormalmice(P<0.05).ConclusionTheexpressionsofMUC1andMUC2areup-regulatedintheOVAsensitizedmice,butits’relationneedfurtherstudy.[

8、Keywords]foodallergy;mucins;ImmunohistochemicalSP-stainingmethod食物过敏(foodallergy，FA)的研究各国已进行数十年，但发生机制仍不清楚。目前机制的研究涉及Th1/Th2细胞反应失衡、肠道局部免疫反应及肠道屏障等内容。其中肠道屏障功能是与FA发生有关的主要原因之一。当感染、应激等干扰肠