姜黄素结合有氧运动对中老年大鼠骨骼肌aktmtor信号通路的影响

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1、ABSTRACTObjective：BydetectingtheratsoleusmuscleIGF一1，changePi3k／Akt／mTORproteinexpressionofitscontenttocellapoptosis，explorecurcumin，constantloadexercise，incrementalExerciseonPi3k／Akt／mTORsignalingpathway．Andcomparingcurcumin，constantloadexercise，incrementalloadexercise，combinedw

2、ithconstantloadexercisecurcumin，curcumincombinedratskeletalmuscleagingeffectofexerciseonincreasingloaddelay．Methods：Thesamebatchof18healthymaleSDratswere60monthsofage(555．61±57．449)，SPF(SpecificpathogenFree，SPF)levelofanimals，housedseparately．Adaptivefeedingforthreedays，wererando

3、mlydividedintosixgroups，wererandomlydividedintocontrolgroup(Controlgroup，BC，n=lO)，curcumin(Curcumingroup，QC，n=lO)，constantloadexercisegroup(Constantloadexercisegroup，CE，n=10)，increasingloadexercisegroup(incrementalExercisegroup，IE，n=lO)，aconstantloadexercise+curcumingroup(constan

4、tloadexercisecurcumingroup，CC，n=10)，incrementalExercise+curcumingroup(IncrementalExercise+curcumingroup，IC，n=lO)．2pointseachtocages，fivepercage．Exercisegroupratsusingtreadmillexercise，theestablishmentofaconstantloadand

5、gtoTable卜2．Slopeof0(equivalenttoExerciseIntensity70％V02max)，fivedaysaweekfortraining，twodaysofrest，trainingatotalof8weeks．AftertheendIII万方数据ofthelast20hoursoftrainingtotakeblood，soleusmusclewasmeasuredIGF一1contentusingE1issamethod，usingWesternBlottingDetectionAkt，Pi3k，mTORprotein

6、expression，andthesituationofskeletalmusclecellapoptosisbyTUNELassay．Results：(1)Muscleapoptosisinratsoleussmallestindexisconstantloadexercisegroup，followedbythecurcumingroup，一．一●●1一apoptotiCindex1Sthelargestgrouplncreaslngload．Comparedwithcurcumingroupandconstantloadgroup，therewas

7、asignificantdifferenceP<0．01：comparedwithcurcuminincreasingloadwassignificantdifferenceP<0．05．(2)comparedwiththegroupandcurcumingroupconstantload，1。ncreasinggloadoadinserumIGF—l—contentwasS111candifference(P

8、tdifference(P<0．05)musclecontentofIGF-Ii