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《木犀草素对对乙酰氨基酚诱导的l02肝细胞损伤的保护作用》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、木犀草素对对乙酰氨基酚诱导的L02肝细胞损伤的保护作用［摘要］探讨木犀草素（luteolin，Lirt）对对乙酰氨基酚（acetaminophen,APAP）诱导的L02肝细胞损伤的保护作用。CCK8法检测Lilt对L02细胞活性的影响；筛选APAP诱导L02细胞损伤的浓度及作用时间；细胞形态学、CCK8实验和流式细胞术检测分析Lut对APAP诱导的L02细胞凋亡的影响；比色法检测细胞上清液中丙二醛（MDA）含量、谷胱甘肽（GSH）及超氧化物歧化酶（SOD）活性；RTPCR检测凋亡相关基因Bax,Bcl2,caspase3的表达。结果显示Lut在2.5〜40umol?L-l不影响L02
2、细胞活性；12mmol?L-lAPAP作用于L02细胞12h可用于建立肝细胞损伤模型。与模型组相比，Lut组细胞状态明显改善，胞体增大，贴壁能力恢复明显；细胞凋亡率明显下降；MDA含量显著下降（P〈0.05或P〈0.01）,GSH和SOD活性显著提高（P〈0.05或P〈0.01）,同吋能够上调Bcl2及下调Bax,caspase3mRNA的表达（P〈0.05或P〈0.01）。该实验证明了Lut对APAP诱导的L02细胞损伤有保护作用，其机制可能与其减轻氧化应激反应和抑制细胞凋亡有关。［关键词］木犀草素；对乙酰氨基酚；L02细胞；氧化应激；细胞凋亡Protectiveeffectsofl
3、utcolinagainstacctaminophcninduccddamageinL02livercellsHELanzhi1,3,MENGYakun2,3,HANYanzhong3,ZHANGZhcnfang3,YINPing2,3,SANGXiuxiu3,XIAOXiaohe3*，BAIZhaofang3*(1.SchoolofPharmacy,HunanUniversityofTraditionalChineseMedicine,Changsha410208,China；2.SchoolofPharmacy,JiangxiUniversityofTraditionalChine
4、seMedicine,Nanchang330004,China；3.ChinaMilitaryInstituteofChineseMedicine,302MilitaryHospital,Beijing100039,China)[Abstract]Thispaperwasaimedtoinvestigatetheprotectiveeffectsofluteolin(Lut)againstacetaminophen(APAP)induceddamageinL02livercells.CCK8wasusedtodetectthecellactivationofL02cellstreate
5、dbydifferentLut.TheconcentrationandtimeofAPAPinducedL02celldamagewasscreened.TheeffectofLutonAPAPinducedapoptosisofL02cellswasdetectedbycellmorphologicalobservation,CCK8assayandflowcytometry.ThecontentsofMDA,GSHandSODactivityincellsupernatantweredetectedbycolorimetricassay.Theexpressionofapoptos
6、isrelatedgenesBax,Bcl2andcaspase3wasdetectedbyRTPCR.TheresultsshowedthatLutin2.540pm()l?L-lrangedoesnotaffecttheactivityofL02cells；12mmol?L-lAPAPincubatedwithL02cell12htoestablishdamagemodel.Comparedwiththemodelgroup,thecellstatusofLutgroupwassignificantlyimproved,thecellbodywasincreased,theadhe
7、renceabilitywasrecovered,andtheapoptosisratewasobviouslydecreased.MDAcontentdecreasedsignificantly(P［Keywords］luteolin；acetaminophen；L02cells;oxydatvestress；apoptosisdoi：10.4268/cjcmm20162224对乙酰氨基酚(acetaminophen,APAP)是一种常见的解
