资源描述：
《环介导等温扩增技术在检测妊娠期女性沙眼衣原体感染中的应用》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、环介导等温扩增技术在检测妊娠期女性沙眼衣原体感染中的应用doi:10.3969/j.issn.1007-614x.2014.22.63摘要目的：评价酶联免疫吸附试验(ELISA)和环介导等温扩增方法(loop-mediatedisothermalamplification,LAMP)在检测妊娠期女性沙眼衣原体(ChlaniydiEietrachomatis,Ct)感染屮的应用价值。方法：根据Genebank公布的Cpn基因序列设计6条LAMP引物(2条内引物，2条外引物，2条环引物)，优化并建立LAMP检测体系。收治
2、Ct感染患者80例，进行LAMP法检测CtDNA,同时用ELISA检测Ct特异性脂多糖抗原。结果:80例患者中,ELISA检测出阳性46例(57.5%),而LAMP检测出阳性56例(70.0%),两者比较差异无统计学意义(P>0.05)o80例患者中有51例服用过大环内酯类抗生素,ELTSA检测出阳性26例(50.9%),而LAMP检测出39例(76.5%),两者比较差异有统计学意义(P<0.05)o结论：LAMP检测泌尿生殖道Ct感染方法简便、快捷，尤其是对服用过抗生素的患儿也具有较高的检出率。关键词沙眼衣原体；环
3、介导等温扩增技术；泌尿生殖道感染ApplicationofloopmediatedisothermalamplificationtechnologyinthedetectionofwomenchlamydiaetrachomatisinfectionduringpregnancyLiuWeil,LiuLiangzhuanl,WangHualil,HeLu2PathogenicBiologyResearchInstituteofNanhuaUniversity(HengyangCity,Hunan),4210011Th
4、eFirstHospitalAffiliatedtoNanhuaUniversity(HengyangCity,Hunan),4210012Abstractobjective：Toevaluatetheapplicationvalueofenzymelinkedimmunosorbentassay(ELISA)andloopmediatedisothermalamplification(LAMP)inthedetectionofwomenchlamydiaetrachomatis(Ct)infectionduring
5、pregnancy.Methods：6LAMPprimers(2innerprimers,2outerprimers,2loopprimers)weredesignedaccordingtothesequenceofCpngenepublishedbyGenebank.LAMPdetectionsystemwasoptimizedandestablished.80caseswithCtwereselected.CtDNAwasdetectedbyLAMP・Ctspecificfatpolysaccharideanti
6、genwasdetectedbyELISA.Results：In80cases,ELISAdetectedthat46cases(57.5%)werepositive,whileLAMPdetectedthat56cases(70%)werepositive,andtherewasnostatisticalsignificance(P>0.05).In80cases,51casesweretreatedwithmacrolideantibiotics；ELISAdetectedthat26cases(50.9%)we
7、repositive,whileLAMPdetectedthat39cases(76.5%)werepositive,andtherewasstatisticalsignificance(P<0.05).Conclusion：LAMPdetectionofurogenitalCtinfectionmethodissimpleandfast,especiallyithashigherdetectionrateforthechildrenwithtakingantibiotics.KeywordsChlamydiaetr
8、achomatis；Loopmediatedisothermalamplificationtechnology；Urinogenitalinfection环介导等温扩增法(loop-mediatedisothermalamplification,LAMP)是一种敏感、特异、方便快捷的核酸扩增技术［1］。因其无需特殊设备，且具有PCR类似的灵敏度
